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Stereometric characterization of kefir microbial films associated with Maytenus rigida extract.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-07-10 , DOI: 10.1002/jemt.23532
Robert Saraiva Matos 1, 2 , Erveton Pinheiro Pinto 2 , Glenda Quaresma Ramos 3 , Marta Duarte da Fonseca de Albuquerque 4 , Henrique Duarte da Fonseca Filho 5
Affiliation  

The aim of this study was to report the first discussion of statistical parameters and possible applications related to the surface of kefir biofilms prepared with Maytenus rigida Mart. extract through micromorphology, using atomic force microscopy (AFM). Four different samples were produced. The reference contained demerara sugar with a concentration of 40 g/L and the others had added extract whose concentrations were 0.25, 0.50, and 0.75 g/L, respectively. AFM was used to image the surface in tapping mode, on square areas of 30 μm × 30 μm. Images were studied by the MountainsMap premium commercial software. The main parameter of the surface, roughness, grew with the increase of the extract concentration up to 0.50 g/L. In addition, asymmetry and kurtosis showed that the biofilm with the best peak distribution was the one with 0.25 g/L of plant extract. The other parameters followed the main results for the surface and were observed for the depth and volume parameters. The power spectrum density spectrum confirmed the reliability of the results for the sample with 0.25 g/L. Therefore, the sample with 0.25 g/L of plant extract produced the best balance in relation to the surface properties. These results showed that the biofilm morphology and microtexture were affected by the incorporation of the plant extract and can be useful to define possible applications.

中文翻译:

与 Maytenusrigida 提取物相关的开菲尔微生物膜的立体表征。

本研究的目的是报告第一次讨论统计参数和可能的应用与用Maytenusrigida制备的开菲尔生物膜表面相关。玛特。使用原子力显微镜 (AFM) 通过显微形态提取。生产了四种不同的样品。参照含有浓度为 40 g/L 的 demerara 糖,其他参照分别添加浓度为 0.25、0.50 和 0.75 g/L 的提取物。AFM 用于以轻敲模式在 30 μm × 30 μm 的方形区域上对表面进行成像。图像由 MountainsMap 高级商业软件研究。表面粗糙度的主要参数随着提取物浓度的增加而增加,达到 0.50 g/L。此外,不对称性和峰度表明,具有最佳峰分布的生物膜是具有 0.25 g/L 植物提取物的生物膜。其他参数遵循表面的主要结果,并观察深度和体积参数。功率谱密度谱证实了 0.25 g/L 样品结果的可靠性。因此,含有 0.25 g/L 植物提取物的样品在表面特性方面产生了最佳平衡。这些结果表明,生物膜形态和微观纹理受植物提取物掺入的影响,可用于定义可能的应用。
更新日期:2020-07-10
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