Exosomes are attracting considerable interest in the cardiovascular field as the wide range of their functions is recognized in acute myocardial infarction (AMI). However, the regulatory role of exosomal long non‐coding RNAs (lncRNAs) in AMI remains largely unclear. Exosomes were isolated from the plasma of AMI patients and controls, and the sequencing profiles and twice qRT‐PCR validations of exosomal lncRNAs were performed. A total of 518 differentially expressed lncRNAs were detected over two‐fold change, and 6 kinds of lncRNAs were strikingly elevated in AMI patients with top fold change and were selected to perform subsequent validation. In the two validations, lncRNAs ENST00000556899.1 and ENST00000575985.1 were significantly up‐regulated in AMI patients compared with controls. ROC curve analysis revealed that circulating exosomal lncRNAs ENST00000556899.1 and ENST00000575985.1 yielded the area under the curve values of 0.661 and 0.751 for AMI, respectively. Moreover, ENST00000575985.1 showed more significant relationship with clinical parameters, including inflammatory biomarkers, prognostic indicators and myocardial damage markers. Multivariate logistic model exhibited positive association of ENST00000575985.1 with the risk of heart failure in AMI patients. In summary, our data demonstrated that circulating exosomal lncRNAs ENST00000556899.1 and ENST00000575985.1 are elevated in patients with AMI, functioning as potential biomarkers for predicting the prognosis of pateints with AMI.

中文翻译：

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急性心肌梗死患者的循环外泌体长链非编码 RNA。

外泌体在心血管领域引起了相当大的兴趣，因为它们的广泛功能在急性心肌梗死 (AMI) 中得到认可。然而，外泌体长链非编码 RNA（lncRNA）在 AMI 中的调节作用仍不清楚。从 AMI 患者和对照组的血浆中分离外泌体，并进行外泌体 lncRNA 的测序谱和两次 qRT-PCR 验证。在2倍变化范围内共检测到518个差异表达的lncRNA，其中6种lncRNAs在AMI患者中显着升高，并被选中进行后续验证。在两次验证中，与对照组相比，AMI 患者的 lncRNA ENST00000556899.1 和 ENST00000575985.1 显着上调。ROC 曲线分析显示，循环外泌体 lncRNA ENST00000556899.1 和 ENST00000575985.1 产生的 AMI 曲线下面积分别为 0.661 和 0.751。此外，ENST00000575985.1与临床参数显示出更显着的相关性，包括炎症生物标志物、预后指标和心肌损伤标志物。多变量逻辑模型显示 ENST00000575985.1 与 AMI 患者心力衰竭风险呈正相关。总之，我们的数据表明，循环外泌体 lncRNA ENST00000556899.1 和 ENST00000575985.1 在 AMI 患者中升高，可作为预测 AMI 患者预后的潜在生物标志物。ENST00000575985.1 与临床参数显示更显着的相关性，包括炎症生物标志物、预后指标和心肌损伤标志物。多变量逻辑模型显示 ENST00000575985.1 与 AMI 患者心力衰竭风险呈正相关。总之，我们的数据表明，循环外泌体 lncRNA ENST00000556899.1 和 ENST00000575985.1 在 AMI 患者中升高，可作为预测 AMI 患者预后的潜在生物标志物。ENST00000575985.1 与临床参数显示更显着的相关性，包括炎症生物标志物、预后指标和心肌损伤标志物。多变量逻辑模型显示 ENST00000575985.1 与 AMI 患者心力衰竭风险呈正相关。总之，我们的数据表明，循环外泌体 lncRNA ENST00000556899.1 和 ENST00000575985.1 在 AMI 患者中升高，可作为预测 AMI 患者预后的潜在生物标志物。