Resveratrol and celastrol loaded Collagen dental implants regulate periodontal ligament fibroblast growth and osteoclastogenesis of bone marrow macrophages,Chemistry & Biodiversity

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Resveratrol and celastrol loaded Collagen dental implants regulate periodontal ligament fibroblast growth and osteoclastogenesis of bone marrow macrophages
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-08-07 , DOI: 10.1002/cbdv.202000295
Ruijie Wang ₁ , Bin Bao ₁ , Chunling Bao ₂ , Shujun Wang ₃ , Saeed Ur Rahman ₄ , Chunyu Hou ₁ , Jeevithan Elango ₁ , Wenhui Wu _{1,

5}

Affiliation

Collagen is widely used for dental therapy in several ways such as films, 3D matrix, and composites, besides traditional Chinese medicine (TCM), has been used in tissue regeneration and wound healing application for centuries. Hence, the present study was targeted for the first time to fabricate collagen film with TCM such as resveratrol and celastrol in order to investigate the human periodontal ligament fibroblasts (HPLF) growth and bone marrow macrophages (BMM) derived osteoclastogenesis. Further, the physicochemical, mechanical and biological activities of collagen‐TCM films crosslinked by glycerol and EDC‐NHS (1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide‐N‐hydroxysulfosuccinimide) were investigated. Collagen film characterization was significantly regulated by the nature of plasticizers like hydrophobic and degree of polarity. Interestingly, the collagen film's denaturation temperature was increased by EDC‐NHS than glycerol. FT‐IR data confirmed the functional group changes due to chemical interaction of collagen with TCM. Morphological changes of HPLF cells cultured in control and collagen films were observed by SEM. Importantly, the addition of resveratrol upregulated the proliferation of HPLF cells, while osteoclastogenesis of BMM cells treated with mCSF‐RANKL was significantly downregulated by celastrol. Accordingly, the collagen‐TCM film could be an interesting material for dental regeneration, and especially it is a therapeutic target to restrain the elevated bone resorption during osteoporosis.

中文翻译：

载有白藜芦醇和 celastrol 的胶原牙种植体调节骨髓巨噬细胞的牙周膜成纤维细胞生长和破骨细胞生成

胶原蛋白以薄膜、3D 基质和复合材料等多种方式广泛用于牙科治疗，除传统中药 (TCM) 外，几个世纪以来一直用于组织再生和伤口愈合应用。因此，本研究首次针对白藜芦醇和 Celastrol 等中药制备胶原膜，以研究人牙周膜成纤维细胞 (HPLF) 的生长和骨髓巨噬细胞 (BMM) 衍生的破骨细胞生成。此外，研究了由甘油和 EDC-NHS（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺-N-羟基磺基琥珀酰亚胺）交联的胶原-TCM 薄膜的理化、机械和生物活性。增塑剂的性质如疏水性和极性程度显着调节胶原膜的特性。有趣的是，与甘油相比，EDC-NHS 提高了胶原膜的变性温度。FT-IR 数据证实了由于胶原与中药的化学相互作用导致的官能团变化。扫描电镜观察对照和胶原膜培养的HPLF细胞形态学变化。重要的是，白藜芦醇的添加上调了 HPLF 细胞的增殖，而用 mCSF-RANKL 处理的 BMM 细胞的破骨细胞生成被 Celastrol 显着下调。因此，胶原-中药薄膜可能是一种有趣的牙齿再生材料，尤其是抑制骨质疏松症期间骨吸收升高的治疗靶点。FT-IR 数据证实了由于胶原与中药的化学相互作用导致的官能团变化。扫描电镜观察对照和胶原膜培养的HPLF细胞形态学变化。重要的是，白藜芦醇的添加上调了 HPLF 细胞的增殖，而用 mCSF-RANKL 处理的 BMM 细胞的破骨细胞生成被 Celastrol 显着下调。因此，胶原-中药薄膜可能是一种有趣的牙齿再生材料，尤其是抑制骨质疏松症期间骨吸收升高的治疗靶点。FT-IR 数据证实了由于胶原与中药的化学相互作用导致的官能团变化。用扫描电镜观察在对照组和胶原膜中培养的HPLF细胞的形态学变化。重要的是，白藜芦醇的添加上调了 HPLF 细胞的增殖，而用 mCSF-RANKL 处理的 BMM 细胞的破骨细胞生成被 Celastrol 显着下调。因此，胶原-中药薄膜可能是一种有趣的牙齿再生材料，尤其是抑制骨质疏松症期间骨吸收升高的治疗靶点。而用 mCSF-RANKL 处理的 BMM 细胞的破骨细胞生成被 Celastrol 显着下调。因此，胶原-中药薄膜可能是一种有趣的牙齿再生材料，尤其是抑制骨质疏松症期间骨吸收升高的治疗靶点。而用 mCSF-RANKL 处理的 BMM 细胞的破骨细胞生成被 Celastrol 显着下调。因此，胶原-中药薄膜可能是一种有趣的牙齿再生材料，尤其是抑制骨质疏松症期间骨吸收升高的治疗靶点。

更新日期：2020-08-07

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