Mycobacteria are difficult to kill due to the complexity of their cell wall. Further, Mycobacterium avium subsp. paratuberculosis (MAP) has one of the more elaborate cell wall compositions of all the mycobacteria. As a working pathogen within a research laboratory setting or as an environmental contaminant shed in the manure from infected animals, MAP is highly resistant to typical disinfectants. In the past, the most successful disinfectants to kill mycobacteria were based upon phenolics, harsh compounds that can break down the lipids within the cell wall. New disinfectants have been developed that are less toxic to the environment, however, it is unknown how well they perform compared to more traditional disinfectants. In the present study, we present comparative data on the utility of a commercial eco-friendly disinfectant, Benefect®, compared to Amphyl®, a phenolic-based disinfectant, and Lysol®, a quaternary ammonium-based disinfectant, to kill MAP in pure culture, tissues, and manure. Results demonstrated that Benefect was highly effective with up to 100% kill of MAP within 30 min in all experiments, paralleling results obtained with Amphyl. Lysol performed the most poorly, requiring longer contact times to kill MAP. These results suggest that natural, nontoxic ingredients can be used to disinfect even hearty pathogens such as MAP effectively, both within the laboratory and on-farm.

分枝杆菌由于其细胞壁的复杂性而难以杀死。此外，鸟分枝杆菌亚种。副结核病（MAP）具有所有分枝杆菌中较为复杂的细胞壁成分之一。MAP作为研究实验室环境中的工作病原体，或作为被感染动物粪便中掉落的环境污染物，对典型的消毒剂具有很高的抵抗力。过去，最成功的杀灭分枝杆菌的消毒剂是基于酚类化合物，酚类化合物是可以分解细胞壁内脂质的苛刻化合物。已经开发出对环境毒性较小的新型消毒剂，但是，与更传统的消毒剂相比，它们的性能如何尚不清楚。在本研究中，我们提供了商业上环保型消毒剂Benefect®与酚类消毒剂Amphyl®和季铵类消毒剂Lysol®杀灭纯MAP的效用的比较数据。文化，组织和肥料。结果表明，在所有实验中，Benefect在30分钟内杀死MAP的效率高达100％，与使用Amphyl获得的结果相似。来苏乐表现最差，需要更长的接触时间才能杀死MAP。这些结果表明，在实验室内和农场内，天然，无毒的成分都可以有效地消毒甚至是强心的病原体，例如MAP。