Semicarbazide (SEM) is a protein-bound nitrofurazone metabolite that is detrimental to human health. Therefore, to ensure food safety, it is necessary to detect SEM in food samples. To this end, we developed a novel electrochemical sensor to detect SEM by using a molecularly imprinted polymer (MIP) as the recognition element. Computer-aided molecular modelling was performed to guide the synthesis of the MIP, and subsequently, MIP/carboxylated single-walled carbon-nanotubes/chitosan (MIP/SWNTs-COOH/CS) was prepared as the sensing platform to develop the electrochemical sensor. The linear range of the sensor was 0.04–7.6 ng mL⁻¹, with a detection limit of 0.025 ng mL⁻¹. The sensor was successfully applied to detect SEM in four different real samples, with recoveries ranging from 83.16% to 93.40%. The results indicated that the fabricated electrochemical sensor can be widely applied to detect SEM in the environment and in agri-food products.

氨基脲（SEM）是与蛋白质结合的呋喃西林的代谢产物，对人体健康有害。因此，为确保食品安全，有必要对食品样品中的SEM进行检测。为此，我们开发了一种新型的电化学传感器，通过使用分子印迹聚合物（MIP）作为识别元素来检测SEM。进行计算机辅助分子建模以指导MIP的合成，随后，准备MIP /羧化单壁碳纳米管/壳聚糖（MIP / SWNTs-COOH / CS）作为开发电化学传感器的传感平台。传感器的线性范围为0.04–7.6 ng mL ^-1，检测极限为0.025 ng mL ^-1。该传感器成功地用于检测四种不同真实样品中的SEM，回收率从83.16％到93.40％。结果表明，所制备的电化学传感器可广泛应用于环境和农产品中的SEM检测。