A solid-phase extraction (SPE) method for enriching and purifying estrogenic disrupting compounds (EDCs) based on the estrogen response element was established. The estrogen receptor was used for molecular recognition, as it specifically binds EDCs. An estrogen response element was used to maintain the activity of the estrogen receptor. High-performance liquid chromatography (HPLC) was used to quantify the EDCs. This method combined with HPLC was applied to detect three kinds of EDCs, such as bisphenol A (BPA), 17β-estradiol, and diethylstilbestrol in a liquid milk matrix, with recoveries of 84.1 ± 8.2% to 113.6 ± 2.9%. The limits of detection and quantification of the established method were 1 × 10^-6 mg·mL⁻¹ and 5 × 10^-6 mg·mL⁻¹. The method was further applied to analyze market samples, including liquid milk, fermented milk, and milk powder. Only BPA was detected from one brand of liquid milk and it was below the regulatory level.

建立了基于雌激素反应元件的富集和纯化雌激素破坏性化合物（EDC）的固相萃取（SPE）方法。雌激素受体用于分子识别，因为它特异性结合EDC。雌激素反应元件用于维持雌激素受体的活性。高效液相色谱（HPLC）用于定量EDC。将该方法与HPLC结合用于检测液态奶基质中双酚A（BPA），17β-雌二醇和己烯雌酚等三种EDC，回收率84.1±8.2％至113.6±2.9％。建立的方法的检出限和定量限为1×10 ^-6 mg·mL ^-1和5×10 ^-6 mg·mL ^-1。该方法进一步应用于分析市场样本，包括液态奶，发酵奶和奶粉。仅从一种品牌的液态奶中检出了BPA，且其含量低于监管水平。