Sodium alginate extracted from Ascophyllum nodosum using conventional chemical extraction (Alg-CCE), microwave-assisted extraction (Alg-MAE), ultrasound-assisted extraction (Alg-UAE), and a combination of both enzyme-assisted and conventional chemical extraction (Alg-EAE/CCE) methods were studied for both physicochemical properties and in vitro prebiotic activity. Alg-EAE/CCE had the highest yield, which was significantly (p < 0.05) higher than Alg-MAE and Alg-UAE. However, total uronic acids were highest in the Alg-UAE and was significantly (p < 0.05) higher than in either Alg-CCE or Alg-MAE. The average molecular weight of all extracts were within the range of 65–215 kDa and had similar dispersity index. There were no significant differences in the mannuronate to guluronate (M/G) ratio of the extracts. Higher guluronic than mannuronic acid absorbance values were observed in the extracts, thus suggesting gelling capabilities because guluronic acid preferentially binds calcium. The growth rate of Lactobacillus delbruecki ssp bulgaricus strain in supplemented growth media at 0.10, 0.30 and 0.50% (w/v), were increased in a dose-dependent manner as compared to an un-supplemented growth medium as control. Similar observations were obtained for the L. casei strain, except at the 0.50% (w/v) inclusion where the growth rate decreased. No significant impacts on short-chain fatty acids production were observed, though pH decreased further in the supplemented sodium alginate growth medium. This study indicated that alginate extracts of A. nodosum were able to show prebiotic responses in two strains of Lactobacillus although there were no significant influences of the extraction methods on the in vitro prebiotic responses.

藻酸钠从提取的泡叶藻使用传统的化学提取（ALG-CCE），微波辅助提取（ALG-MAE），超声辅助提取（ALG-UAE），和两者的组合酶辅助和常规化学萃取（ALG -EAE / CCE）方法的理化性质和体外研究益生元活性。Alg-EAE / CCE的产量最高，比Alg-MAE和Alg-UAE显着高（p <0.05）。但是，总糖醛酸在Alg-UAE中最高，并且显着（p <0.05）高于Alg-CCE或Alg-MAE。所有提取物的平均分子量在65–215 kDa范围内，并且具有相似的分散指数。提取物中的甘露糖酸酯与古洛糖酸酯（M / G）比例无显着差异。在提取物中观察到比甘露糖醛酸更高的古洛糖醛酸吸收值，因此暗示了胶凝能力，因为古洛糖醛酸优先结合钙。增速乳杆菌delbruecki SSP保加利亚与未添加生长培养基作为对照相比，添加生长培养基中浓度为0.10％，0.30％和0.50％（w / v）的细菌菌株以剂量依赖性方式增加。对于干酪乳杆菌菌株，获得了相似的观察结果，除了在0.50％（w / v）的夹杂物中，生长速率下降。尽管在补充的藻酸钠生长培养基中pH进一步降低，但未观察到对短链脂肪酸生产的显着影响。这项研究表明，结节曲霉的藻酸盐提取物能够在两种乳酸杆菌菌株中显示益生元响应，尽管提取方法对体外益生元响应没有显着影响。