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Lipid production by Cryptococcus curvatus growing on commercial xylose and subsequent valorization of fermentation waste-waters for the production of edible and medicinal mushrooms
Biochemical Engineering Journal ( IF 3.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.bej.2020.107706
Panagiota Diamantopoulou , Nikolaos G. Stoforos , Evangelos Xenopoulos , Dimitris Sarris , Dimitrios Psarianos , Antonios Philippoussis , Seraphim Papanikolaou

Abstract A rarely studied Cryptoccus curvatus strain (viz. NRRL Y-1511) was cultivated in shake-flask submerged nitrogen-limited experiments, with commercial xylose being employed as substrate and has been proved as quite appropriate for total biomass, lipid and endopolysaccharide production. A batch bioreactor trial compared to the equivalent shake-flask experiment showed larger amounts of total lipid and biomass produced. Total dry biomass of 26.1 g/L containing c. 30 % of lipids was synthesized. Endopolysaccharides in relatively high concentrations were synthesized in the early growth stages decreasing afterwards in favor of storage lipid synthesis. Lipid production bioprocess was successfully simulated by a modified Velruyten-Aggelis model and the optimized parameter values were found to be similar to those experimentally measured. Lipids contained mostly neutral fractions (triacylglycerols) and were rich in the fatty acid Δ9C18:1, constituting a suitable starting material for the synthesis of high-quality biodiesel. Within the frame of bio-refinery, lipid fermentation waste-waters were used as maceration waters for a novel mushroom cultivation process of Pleurotus ostreatus, Lentinula edodes and Ganoderma adspersum edible and medicinal mushrooms. These waste-waters were used for first time in mushroom cultivation process demonstrating positive effect on laccase and sporophore production by these mushroom species.

中文翻译:

用商业木糖生长的弯曲隐球菌生产脂质,随后对发酵废水进行增值,用于生产食用和药用蘑菇

摘要 一种很少研究的弯曲隐球菌菌株(即 NRRL Y-1511)在摇瓶浸没氮限制实验中培养,使用商业木糖作为底物,并已被证明非常适合总生物量、脂质和内多糖的生产。与等效的摇瓶实验相比,分批生物反应器试验显示产生的总脂质和生物量更大。总干生物量为 26.1 g/L,含 c。合成了 30% 的脂质。在早期生长阶段合成相对高浓度的内多糖,之后逐渐减少,有利于储存脂质的合成。通过改进的 Velruyten-Aggelis 模型成功模拟了脂质生产生物过程,发现优化的参数值与实验测量值相似。脂质主要包含中性组分(甘油三酯)并富含脂肪酸 Δ9C18:1,是合成高质量生物柴油的合适起始材料。在生物炼制框架内,脂质发酵废水被用作浸渍水,用于平菇、香菇和灵芝食药用蘑菇的新型蘑菇栽培工艺。这些废水首次用于蘑菇栽培过程,证明了这些蘑菇物种对漆酶和孢子体产生的积极影响。脂质发酵废水被用作浸渍水,用于平菇、香菇和灵芝食用和药用蘑菇的新型蘑菇栽培过程。这些废水首次用于蘑菇栽培过程,证明了这些蘑菇物种对漆酶和孢子体产生的积极影响。脂质发酵废水被用作浸渍水,用于平菇、香菇和灵芝食用和药用蘑菇的新型蘑菇栽培过程。这些废水首次用于蘑菇栽培过程,证明了这些蘑菇物种对漆酶和孢子体产生的积极影响。
更新日期:2020-10-01
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