Abstract In this study, conjugated linoleic acid (CLA) was produced using Lactobacillus plantarum in biofilm reactors with different carbon sources (glucose, lactose or a combination of glucose-lactose (1:1)). Presence of metal support in reactors resulted in high biofilm formation by L. plantarum. Two isomers of CLA were identified in fermentation broths; a) cis-9, trans-11 C18:2 (CLA1) as the predominant isomer (22.12 μg/mL), and b) trans-9, trans-11 C18:2 (CLA2) (14.96 μg/mL). Further analyses showed that depending on the carbon source used for the growth of L. plantarum in biofilm reactors, the amount of biomass and CLA production changed. Results indicated that by using combined lactose-glucose (1:1) as carbon source in biofilm reactors, the highest biomass (3.66 g/L) and the maximum concentration of CLA (37.08 μg/mL) were obtained. Scanning electron microscopy of the support used within the fermentation showed that the highest biofilm accumulation on the metal support was associated with the broth supplemented with combined glucose-lactose. This study suggested a high capacity of immobilized L. plantarum on the metal support to produce high quantity of CLA isomers with the use of combined glucose-lactose.

中文翻译：

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金属载体和不同碳源对植物乳杆菌生产CLA的影响

摘要 在本研究中，使用植物乳杆菌在具有不同碳源（葡萄糖、乳糖或葡萄糖-乳糖的组合 (1:1)）的生物膜反应器中生产共轭亚油酸 (CLA)。反应器中金属载体的存在导致植物乳杆菌形成大量生物膜。在发酵液中鉴定出两种 CLA 异构体；a) cis-9, trans-11 C18:2 (CLA1) 作为主要异构体 (22.12 μg/mL)，和 b) trans-9, trans-11 C18:2 (CLA2) (14.96 μg/mL)。进一步的分析表明，根据用于生物膜反应器中植物乳杆菌生长的碳源，生物量和 CLA 产量会发生变化。结果表明，在生物膜反应器中，以乳糖-葡萄糖（1:1）组合为碳源，获得了最高的生物量（3.66 g/L）和最高的CLA浓度（37.08 μg/mL）。发酵过程中使用的支持物的扫描电子显微镜显示，金属支持物上最高的生物膜积累与补充有组合葡萄糖-乳糖的肉汤有关。该研究表明金属载体上的固定化植物乳杆菌具有高容量，可通过使用组合葡萄糖-乳糖产生大量 CLA 异构体。