Abstract In order to examine the effects of different stocking densities and culture methods on the growth, food intake and expression of related genes in Haliotis discus hannai ♀ × H. fulgens♂ hybrid abalones, juvenile abalones were placed into three treatment groups of different stocking densities (400, 600, 800 ind/m2) and cultured using two different methods, including suspension cages and bottom sowing. There were three replicates of each experimental group (stocking density and culture method) and the experiment lasted for 180 days. The results showed that the survival rate, specific growth rate of shell length and body weight, food intake and feed conversion efficiency of the abalones cultured in suspension cages were significantly lower than those in the bottom sown group. For abalones cultured by bottom sowing, there was no significant difference between the specific growth rate of shell length and body weight, feed conversion efficiency, α-amylase, cellulase activity and the expression levels of Hdlam on each sampling occasion for the 400 and 600 ind/m2 treatments. However, these parameters were significantly higher in both the 400 and 600 ind/m2 treatments compared with the 800 ind/m2 treatment. When the stocking density was increased to 800 ind/m2, the food intake and the expression levels of Hdaly, Hdcel, Hdamyl of the abalones cultured in both culture methods were significantly lower than the 400 and 600 ind/m2 treatments. This indicates that even with sufficient food supply, some abalones were unable to capture food due to the constraints of the culture space and the feeding method used by abalones, resulting in reduced growth. The findings of this research suggest that the growth and food intake of abalones can be promoted and production can be improved at a stocking density of 600 ind/m2.

中文翻译：

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放养密度对Haliotis discus hannai ♀×H. fulgens♂杂种成活生长的影响

摘要 为研究不同放养密度和培养方式对Haliotis discus hannai ♀ × H. fulgens♂杂交鲍鱼生长、摄食量及相关基因表达的影响，将幼鲍分为3个不同放养密度的处理组。 (400、600、800 ind/m2) 并使用两种不同的方法进行培养，包括悬笼和底播。每个试验组设3个重复（放养密度和养殖方式），试验持续180天。结果表明，悬浮网箱养殖鲍鱼的成活率、壳长和体重比生长率、摄食量和饲料转化率均显着低于底播组。对于底播养殖的鲍鱼，400 和 600 ind/m2 处理的壳长和体重比增长率、饲料转化效率、α-淀粉酶、纤维素酶活性和 Hdlam 的表达水平在每个采样时间之间没有显着差异。然而，与 800 ind/m2 处理相比，400 和 600 ind/m2 处理的这些参数显着更高。当放养密度增加到800 ind/m2时，两种养殖方式培养的鲍鱼摄食量和Hdaly、Hdcel、Hdamyl的表达水平均显着低于400和600 ind/m2处理。这表明，即使食物供应充足，部分鲍鱼也因养殖空间和鲍鱼饲养方式的限制而无法捕获食物，导致生长速度下降。