The capture and storage of CO₂ has been a critical strategy towards coping with global warming in recent years. Mineral carbonation is gaining much attention as a novel method of CO₂ storage owing to its stability and safety. In this study, the mechanism and effect of carbonation of heat-activated serpentine [Mg₃Si₂O₅(OH)₄] driven by cyanobacteria were explored, taking Microcystis aeruginosa PCC7806 as an example. The serpentine was heated at different temperatures, and thermal decomposition of serpentine was characterized by simultaneous thermogravimetry and differential thermal analysis (TG-DTA), X-ray diffraction (XRD) and scanning electron microscope (SEM). The pH, ion concentrations, mineral composition and surface morphology in the process of interactions between the algae cells and heat-activated serpentine were analyzed. The results show that the serpentine can be transformed to forsterite after heat treatment at over 614 °C, and the medium for the addition of heat-activated serpentine at over 614 °C did not allow algae growth. The algae grew well in BG11 liquid medium with 400 °C heat-activated serpentine, and the pH of the culture liquid reached 10.2–10.3, which is in favor of the carbonation of serpentine. The analysis results show the carbonation of trace serpentine. Moreover, the carbonation effect of 400 °C heat-activated serpentine driven by the algae in a solid medium was the most significant, and the carbonate content of serpentine increased by 75.21% as detected by chemical analysis. XRD, SEM, and energy dispersive spectrometer (EDS) results indicate that amorphous CaMg(CO₃)₂ and MgCO₃ were formed on the surface of serpentine driven by the algae. This study advances our understanding of the biological mechanism of serpentine carbonation, and provides a new way to fully exploit serpentine resources in carbon sequestration.

近年来，CO ₂的捕获和储存一直是应对全球变暖的关键策略。作为一种新型的CO ₂储存方法，矿物碳酸化由于其稳定性和安全性而倍受关注。本研究以铜绿微囊藻为研究对象，探讨了由蓝细菌驱动的热活化蛇纹石[Mg ₃ Si ₂ O ₅（OH）₄ ]的碳酸化机理及其作用。以PCC7806为例。将蛇纹石在不同温度下加热，并通过同时热重分析和差示热分析（TG-DTA），X射线衍射（XRD）和扫描电子显微镜（SEM）来表征蛇纹石的热分解。分析了藻类细胞与热活化蛇纹石相互作用过程中的pH，离子浓度，矿物质组成和表面形态。结果表明，在超过614°C的温度下进行热处理后，蛇纹石可转变为镁橄榄石，并且在超过614°C的温度下添加热活化蛇纹石的介质不允许藻类生长。在具有400°C热活化蛇纹石的BG11液体培养基中，藻类生长良好，培养液的pH值达到10.2–10.3，这有利于蛇纹石的碳酸化。分析结果显示痕量蛇纹石碳酸化。此外，藻类在固体介质中驱动的400°C热活化蛇纹石的碳酸化作用最为显着，化学分析发现蛇纹石的碳酸盐含量增加了75.21％。XRD，SEM和能量色散仪（EDS）结果表明，非晶态CaMg（CO₃）₂和MgCO ₃在藻类驱动的蛇纹石表面形成。这项研究提高了我们对蛇纹石碳酸化的生物学机制的理解，并为在碳固存中充分利用蛇纹石资源提供了新的途径。