Percutaneous collagen induction (PCI) is an alternative treatment for skin dysfunctions, it aims to stimulate collagen production by encouraging normal wound healing that occurs after any trauma by inducing microlesions; also it may be potentiated with the association with drugs such as hyaluronic acid (HA). Our objective was to evaluate the effects of PCI associated with hyaluronic acid (0.9%) on inflammatory process, oxidative stress, and collagen production in rat epidermis. For the study, 36 adult Wistar rats were randomly divided into 6 groups (n = 6): Control; PCI 0.5; PCI 1.0; HA; PCI 0.5 + HA; and PCI 1.0 + HA. The animals were anesthetized, trichotomized, and the application of therapies was performed once; After 7 days, the animals were euthanized for removal of the skin region. Levels of pro-inflammatory (IL1, IL6, TNFα), anti-inflammatory (IL4 and IL10) cytokines and growth factors (FGF, TGFβ) were evaluated, besides oxidative stress parameters and histological analysis. In combination groups, there is a decrease in TNFα compared with the control and PCI groups in contrast to a significant increase in anti-inflammatory cytokines and growth factors. Oxidant and oxidative damage levels showed a significant decrease in PCI + HA groups in relation to PCI groups while antioxidant defense increased in PCI + HA groups compared with the control group. The number of fibroblasts was increased in the PCI 1.0 group in relation to the control, HA, and PCI 0.5. The number of blood vessels and collagen area was increased in groups PCI and PCI + HA compared with the HA group. We conclude that the combination of PCI with HA is able to accelerate the acute inflammatory process, reducing its deleterious effects and anticipating the chronic response, contributing to tissue repair.

经皮胶原诱导（PCI）是皮肤功能障碍的一种替代疗法，它旨在通过诱导微损伤促进任何创伤后发生的正常伤口愈合来刺激胶原蛋白的产生；也可能与透明质酸 (HA) 等药物的关联性增强。我们的目的是评估与透明质酸（0.9%）相关的 PCI 对大鼠表皮炎症过程、氧化应激和胶原蛋白生成的影响。本研究将 36 只成年 Wistar 大鼠随机分为 6 组（n = 6): 控制; PCI 0.5；PCI 1.0；哈; PCI 0.5 + 高可用性；和 PCI 1.0 + HA。将动物麻醉，三分法，并进行一次治疗应用；7天后，将动物安乐死以去除皮肤区域。除了氧化应激参数和组织学分析外，还评估了促炎（IL1、IL6、TNFα）、抗炎（IL4 和 IL10）细胞因子和生长因子（FGF、TGFβ）的水平。在联合组中，与对照组和 PCI 组相比，TNFα 减少，而抗炎细胞因子和生长因子显着增加。与 PCI 组相比，PCI + HA 组的氧化和氧化损伤水平显着降低，而与对照组相比，PCI + HA 组的抗氧化防御增加。与对照组、HA 和 PCI 0.5 相比，PCI 1.0 组的成纤维细胞数量增加。与HA组相比，PCI组和PCI+HA组的血管数量和胶原面积增加。我们得出结论，PCI 与 HA 的组合能够加速急性炎症过程，减少其有害影响并预测慢性反应，有助于组织修复。