Introducing lysozyme (Lys) to dopamine-modified poly (γ-glutamic acid) (PGA-DA), the self-assembled colloidal nanoparticles (Lys/PGA-DA) were obtained through the electrostatic, hydrogen bonding and hydrophobic interactions. After mixing vigorously colloidal nanoparticles and white oil, o/w gel-like emulsion formed. The influences of ionic strength on the properties and emulsifying performances of colloidal nanoparticles and the activity of Lys in emulsions were investigated in detail. Scanning electron microscopy (SEM) characterization and rheological measurements demonstrated that emulsifying properties of colloidal nanoparticles were significantly improved with increasing salt concentration. Moreover, fluorescence and circular dichroism (CD) measurements showed that the addition of salt might induce conformational changes of Lys in colloidal nanoparticles resulting in a more ordered secondary structure, thereby leading to an increase in enzyme activity. In vitro cytotoxicity of emulsions revealed that emulsions showed a low cytotoxicity and a high bioactivity. All results verified that the obtained emulsion could have potential application in food, pharmaceutical, and cosmetic fields. Graphical abstract Graphical abstract

中文翻译：

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离子强度对含溶菌酶自组装胶体纳米粒子稳定的凝胶状Pickering乳液的影响

将溶菌酶（Lys）引入多巴胺修饰的聚（γ-谷氨酸）（PGA-DA）中，通过静电、氢键和疏水相互作用获得自组装胶体纳米粒子（Lys/PGA-DA）。将胶体纳米颗粒与白油剧烈混合后，形成了水包油凝胶状乳液。详细研究了离子强度对纳米胶体性质和乳化性能以及乳液中赖氨酸活性的影响。扫描电子显微镜 (SEM) 表征和流变学测量表明，随着盐浓度的增加，胶体纳米颗粒的乳化性能得到显着改善。而且，荧光和圆二色性 (CD) 测量表明，添加盐可能会引起胶体纳米粒子中赖氨酸的构象变化，从而导致更有序的二级结构，从而导致酶活性增加。乳液的体外细胞毒性表明，乳液具有低细胞毒性和高生物活性。所有结果均证实所获得的乳液在食品、医药和化妆品领域具有潜在的应用价值。图形摘要图形摘要 和化妆品领域。图形摘要图形摘要 和化妆品领域。图形摘要图形摘要