We developed a method of reproducing malignant process in the lungs of rats with stimulation by chronic neurogenic pain. In white outbred male rats, chronic neurogenic pain was modeled by bilateral ligation of the sciatic nerves and in 45 days, a suspension of M1 sarcoma cells (10 6 cells/liter in 0.3 ml physiological saline) was injected into the subclavian vein. In almost all rats receiving transplantation of tumor cells against the background of chronic neurogenic pain, tumor foci in the lungs were detected in 1.5-2.0 months and led to the death of the animals.

中文翻译：

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大鼠肺慢性疼痛刺激恶性生长的方法

我们开发了一种在慢性神经源性疼痛刺激下在大鼠肺中再现恶性过程的方法。在白色远交雄性大鼠中，通过双侧坐骨神经结扎模拟慢性神经源性疼痛，并在 45 天后将 M1 肉瘤细胞悬液（10 6 细胞/升在 0.3 ml 生理盐水中）注射到锁骨下静脉。在几乎所有以慢性神经源性疼痛为背景接受肿瘤细胞移植的大鼠中，肺中的肿瘤病灶在 1.5-2.0 个月内被检测到并导致动物死亡。