We studied the effects and mechanisms of action of NaHS, an H 2 S donor, on isolated phenylephrine-precontracted bovine mesenteric lymph nodes. NaHS induced concentration-dependent relaxation of lymph nodes. Removal of the endothelium reduced, but did not abolish the relaxing effect of NaHS. The relaxing effect was reduced by NO synthase inhibitor L-NAME, soluble guanylate cyclase inhibitor ODQ, ATP-sensitive K + channel blocker glibenclamide, and a combination charybdotoxin+apamin (blockers of small- and intermediate-conductance Ca 2+ -activated K + channels). Thus, the relaxing effect of H 2 S on lymph nodes is mediated by several parallel mechanisms. H 2 S induces relaxation of LN and modulates the rate of lymph transport, thereby affecting the development of immune processes in the body.

中文翻译：

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硫化氢对离体牛肠系膜淋巴结的松弛作用

我们研究了 NaHS（一种 H 2 S 供体）对分离的去氧肾上腺素预收缩的牛肠系膜淋巴结的作用和机制。NaHS 诱导淋巴结的浓度依赖性松弛。去除内皮减少，但没有消除 NaHS 的松弛作用。NO 合酶抑制剂 L-NAME、可溶性鸟苷酸环化酶抑制剂 ODQ、ATP 敏感的 K + 通道阻滞剂格列本脲和组合 charybdotoxin + apamin（小和中电导 Ca 2+ 激活的 K +频道）。因此，H 2 S 对淋巴结的松弛作用是由几种平行机制介导的。H 2 S 诱导 LN 松弛并调节淋巴转运速率，从而影响体内免疫过程的发展。