Objective The present work aimed to investigate the potential utility of Sam68 protein as a prognostic marker in lung cancer. Then an electrochemical immunosensor is fabricated that is sufficiently sensitive to detect Sam68. Results Analysis of stage-specific Lung cancer microarray data shows that differential expression of Sam68 is associated with cancer stage and monotonically increases from early tumor stage to advanced metastatic stage. Moreover, the higher expression of Sam68 results in reduced survival of lung cancer patients. Based on these observations, an electrochemical immunosensor was developed for the quantification of Sam68 protein. The target protein was captured by the Anti-Sam68 antibody that was immobilized on the modified Glassy carbon electrode. The stepwise assembly process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. This fabricated immunosensor displayed good analytical performance in comparison to commercial ELISA kit with good sensitivity, lower detection limit (LOD) of 10.5 pg mL −1 , and wide linear detection range from 1 to 5 μg mL −1 . This method was validated with satisfactory detection of Sam68 protein in lung adenocarcinoma cell line, NCI-H23. Besides, spike and recovery assay reconfirm that the sensor can precisely quantify Sam68 protein in a complex physiological sample. Conclusion We conclude Sam68 as a valuable prognostic biomarker for early detection of lung cancer. Moreover, we report the first study on the development of an electrochemical immunosensor for the detection of Sam68. The fabricated immunosensor exhibit excellent analytical performance, which can accurately predict the lung cancer patient pathological state.

中文翻译：

---

基于抗体的生物传感器检测致癌剪接因子 Sam68 用于肺癌诊断

目的目前的工作旨在研究 Sam68 蛋白作为肺癌预后标志物的潜在效用。然后制造出足够灵敏的电化学免疫传感器来检测 Sam68。结果阶段特异性肺癌微阵列数据分析表明，Sam68的差异表达与癌症阶段相关，并且从早期肿瘤阶段到晚期转移阶段单调增加。此外，Sam68 的较高表达导致肺癌患者的存活率降低。基于这些观察，开发了一种用于 Sam68 蛋白定量的电化学免疫传感器。目标蛋白被固定在修饰玻璃碳电极上的 Anti-Sam68 抗体捕获。逐步组装过程的特点是循环伏安法和电化学阻抗谱。与商业ELISA试剂盒相比，这种制造的免疫传感器显示出良好的分析性能，具有良好的灵敏度、10.5 pg mL -1 的检测下限（LOD）和1至5 μg mL -1 的宽线性检测范围。该方法通过在肺腺癌细胞系 NCI-H23 中对 Sam68 蛋白的满意检测得到验证。此外，spike 和 recovery 检测再次证实该传感器可以精确定量复杂生理样品中的 Sam68 蛋白。结论 我们认为 Sam68 是肺癌早期检测的有价值的预后生物标志物。此外，我们报告了关于开发用于检测 Sam68 的电化学免疫传感器的第一项研究。