A feeding trial was conducted to evaluate the effects of the fishmeal level in the diet of the oriental river prawn, Macrobrachium nipponense, on growth performance and gene expression related to protein and lipid metabolism. Six isonitrogenous (41%) and isolipid (8.5%) diets were designed with decreasing fishmeal (FM) levels from 35% to 30%, 25%, 20%, 15%, and 10%. Each diet was fed to three tanks of 50 prawns for 9 weeks. Decreasing FM levels in the diet from 35 to 25% had no adverse effects on growth performance and feed utilization. However, growth performance and feed utilization were significantly decreased when the FM level in the diet was decreased to below 20%. FM reduction in the diet of oriental river prawn had no effect on survival and whole-body composition with the exception of lipid content. Decreasing the FM level to below 25% upregulated the relative gene expression of TOR, ATF4, and ATF3 in the hepatopancreas. The mRNA levels of ATF4 and ATF3 were upregulated in the muscle of those fed the diet with 25% FM. Reduction of the FM level in the diet to less than 20% increased the relative expression of fatty acid synthesis genes such as FAS, ACC, and G6PDH, and increased the relative gene expression of CPT1. Taken together, a 25% FM level in the diet was found to be optimal for M. nipponense.

进行了一项饲喂试验，以评估鱼粉水平对东方河虾日本沼虾的饮食的影响，关于与蛋白质和脂质代谢有关的生长性能和基因表达。设计了六种等氮（41％）和等脂（8.5％）日粮，使鱼粉（FM）含量从35％降低到30％，25％，20％，15％和10％。每种饮食都喂入三个50只虾的罐中，持续9周。日粮中的FM水平从35％降低到25％对生长性能和饲料利用率没有不利影响。但是，当日粮中的FM含量降至20％以下时，生长性能和饲料利用率将大大降低。FM减少东方河虾的饮食，除了脂质含量外，对存活率和全身成分没有影响。将FM水平降低至25％以下可上调肝胰腺中TOR，ATF4和ATF3的相对基因表达。在饮食中添加25％FM的肌肉中，ATF4和ATF3的mRNA水平上调。日粮中的FM含量降至20％以下可增加脂肪酸合成基因（例如FAS，ACC和G6PDH）的相对表达，并增加CPT1的相对基因表达。两者合计，发现饮食中FM含量为25％最适合日本烟草。