In this study, we developed a procedure for assembling hepatic microstructures into tube shapes using magnetic self-assembly for in vitro 3D micro-tissue fabrication. To this end, biocompatible hydrogels, which have a toroidal shape, were made using the micro-patterned electrodeposition method. Ferrite particles were used to coat the fabricated toroidal hydrogel microcapsules using a poly-L-lysine membrane. The microcapsules were then magnetized with a 3 T magnetic field, and assembled using a magnetic self-assembly process. During electrodeposition, hepatic cells were trapped inside the microcapsules, and they were cultured to construct tissue-like structures. The magnetized toroidal microstructures then automatically assembled to form tube structures. Shaking was used to enhance the assembly process, and the shaking speed was experimentally optimized to achieve the high-speed assembly of longer tube structures. The flow velocity inside the dish during shaking was measur...

中文翻译：

---

用于微组织制造的环形肝微结构的磁性自组装。

在这项研究中，我们开发了一种使用磁性自组装将肝脏微结构组装成管状的程序，用于体外 3D 微组织制造。为此，使用微图案电沉积方法制造了具有环形形状的生物相容性水凝胶。铁氧体颗粒用于使用聚-L-赖氨酸膜涂覆制造的环形水凝胶微胶囊。然后将微胶囊用 3 T 磁场磁化，并使用磁性自组装工艺组装。在电沉积过程中，肝细胞被困在微囊内，并被培养以构建组织样结构。磁化的环形微结构然后自动组装以形成管结构。振动被用来增强装配过程，并对振动速度进行了实验优化，以实现较长管结构的高速组装。摇动过程中培养皿内的流速被测量...