This study aimed to analyze the complete genome sequence of the butenyl-spinosyn-producing strain Saccharopolyspora sp. ASAGF58, isolated from Zhejiang province. PacBio RS II sequencing platform with single-molecule real-time technology was used to obtain the complete genome sequence of Saccharopolyspora sp. ASAGF58. Gene prediction and annotation analysis were carried out through several software and databases. The antiSMASH online server was used to evaluate the secondary metabolite potential of strain ASAGF58. The whole genome of Saccharopolyspora sp. ASAGF58 is 8,190,340 bp divided into one chromosome of 8,044,361 bp with a GC content of 68.1% and a plasmid of 145,979 bp with a GC content of 64.6%. A total of 7486 coding sequences, 15 rRNA genes, 61 tRNA genes, 41 miscRNA genes, and 1 tmRNA gene were predicted. The domains encoded by one of the type I polyketide synthase (T1PKS) gene clusters have 91% similarity with those encoded by a spinosad biosynthetic gene cluster from Saccharopolyspora spinosa. In addition, antiSMASH results predicted that the strain also contains the biosynthetic gene clusters for the synthesis of ectoine, geosmin, and erythreapeptin. Our data revealed the complete genome sequence of a new isolated butenyl-spinosyn-producing strain. This work will provide some methods, from genetics to biotechnology and biochemistry, aimed at the production improvement of butenyl-spinosyns.

中文翻译：

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丁烯基多杀菌素生产的完整基因组序列糖多孢菌株ASAGF58

这项研究旨在分析生产丁烯基多杀菌素菌株Saccharopolyspora sp的完整基因组序列。ASAGF58，从浙江省分离。使用具有单分子实时技术的PacBio RS II测序平台来获得Saccharopolyspora sp的完整基因组序列。ASAGF58。基因预测和注释分析是通过几种软件和数据库进行的。antiSMASH在线服务器用于评估菌株ASAGF58的次生代谢产物潜力。Saccharopolyspora sp。的整个基因组。ASAGF58为8,190,340 bp，分为一个染色体8,044,361 bp，GC含量为68.1％，质粒为145,979 bp，GC含量为64.6％。总共预测了7486个编码序列，15个rRNA基因，61个tRNA基因，41个miscRNA基因和1个tmRNA基因。由I型聚酮化合物合酶（T1PKS）基因簇之一编码的结构域与由棘糖多孢菌的多刺生物合成基因簇编码的结构域具有91％的相似性。此外，antiSMASH结果预测该菌株还包含用于合成外皮素，土臭素和赤肽的生物合成基因簇。我们的数据揭示了新分离出的丁烯基多杀菌素生产菌株的完整基因组序列。这项工作将提供一些方法，从遗传学到生物技术和生物化学，旨在改善丁烯基多杀菌素的生产。antiSMASH结果预测该菌株还包含用于合成植物素，土臭素和赤藓肽的生物合成基因簇。我们的数据揭示了新分离出的丁烯基多杀菌素生产菌株的完整基因组序列。这项工作将提供一些方法，从遗传学到生物技术和生物化学，旨在改善丁烯基多杀菌素的生产。antiSMASH结果预示该菌株还含有用于合成外皮素，土臭素和赤藓肽的生物合成基因簇。我们的数据揭示了新分离出的丁烯基多杀菌素生产菌株的完整基因组序列。这项工作将提供一些方法，从遗传学到生物技术和生物化学，旨在改善丁烯基多杀菌素的生产。