Application of HPLC-Q/orbitrap MS in the detection and identification of anticancer constituents in ethyl acetate components from Hedyotis diffusa.,Analytical Methods

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Application of HPLC-Q/orbitrap MS in the detection and identification of anticancer constituents in ethyl acetate components from Hedyotis diffusa.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-07-09 , DOI: 10.1039/d0ay00531b
Xi Chen ₁ , Baojin Zhao , Mengjiao Zhang , Min Dong , Yunlan Li , Qingshan Li

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A feasible analytical method based on high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (HPLC-Q/orbitrap MS) has been established for the identification and characterization of anticancer constituents in ethyl acetate components from Hedyotis diffusa in our work. The mass spectrometer provided significant fragment information both in the full MS scan and data-dependent MS² modes. Sixty-two possible compounds were analyzed and identified from the above results. Of the above 62 compounds, 12 have good separation in the positive ion mode, and 27 compounds have good separation in the anion mode. Currently, 39 have been reported in the literature related to the chemical composition of the plant, while the other 23 of the 62 compounds have not been reported. Fifteen tentatively identified compounds were given detailed descriptions. Four representative compounds from the ethyl acetate extract among the fifteen were actually isolated in good yield with silica gel column chromatography. Meanwhile, their structures were unambiguously identified as scopoletin, vanillic acid, p-coumaric acid and E-6-O-p-coumaroyl scandoside methyl ester using ¹H NMR, ¹³C NMR, IR and UV spectroscopy. The purity of the ethyl acetate extracts, the total flavonoids, from Hedyotis diffusa could reach 65.6%. The inhibitory effect on HepG-2 cells of the total flavonoids was up to 66.2 ± 2.7%. Amentoflavone, quercetin, naringenin, and rutin were selected from the total flavonoids. The amentoflavone and quercetin had better hepatoprotective activity. The inhibition rates of 500 μmol L⁻¹ amentoflavone and quercetin on HepG-2 cells could reach 56.2 ± 8.1% and 78.0 ± 9.3%, respectively. The EtOAc extracts could induce apoptosis in HepG2 cells by blocking the cell cycle in the G0/G1 phase. To the best of our knowledge, this is the first time that Q-orbitrap HRMS was applied to detect potential anticancer compounds in Hedyotis diffusa. This analytical method proved to be a feasible approach for the rapid detection of the potential anticancer compounds from Hedyotis diffusa.

中文翻译：

HPLC-Q / orbitrap MS在检测和鉴定白花蛇舌草乙酸乙酯成分中抗癌成分中的应用。

已建立抗癌活性成分的乙酸乙酯组分从鉴定和表征基于高性能液相色谱-四极/静电场轨道阱高分辨率质谱一种可行的分析方法（HPLC-Q /轨道阱MS）白花蛇舌草在我们的工作。质谱仪在完整的MS扫描和与数据相关的MS ^2中都提供了重要的碎片信息模式。根据以上结果分析并鉴定了62种可能的化合物。在上述62种化合物中，有12种在正离子模式下具有良好的分离，而27种化合物在阴离子模式下具有良好的分离。目前，在文献中已经报道了与植物化学成分有关的39种化合物，而62种化合物中的23种化合物尚未被报道。给出了15种初步鉴定的化合物的详细说明。实际上，用硅胶柱色谱从乙酸乙酯提取物中分离出四种代表性化合物，收率很高。同时，它们的结构被明确地鉴定为pole胆碱，香草酸，对香豆酸和E -6- Op^-1 H NMR，¹³ C NMR，IR和UV光谱法测定-香豆酰基Scandoside甲酯。白花蛇舌草乙酸乙酯提取物总黄酮的纯度可达到65.6％。总黄酮对HepG-2细胞的抑制作用高达66.2±2.7％。从总黄酮中选择了黄酮，槲皮素，柚皮素和芦丁。黄酮和槲皮素具有更好的保肝活性。500μmolL ^-1的抑制率HepG-2细胞上的黄酮和槲皮素分别可达到56.2±8.1％和78.0±9.3％。EtOAc提取物可通过阻断G0 / G1期的细胞周期来诱导HepG2细胞凋亡。据我们所知，这是Q-orbitrap HRMS首次用于检测白花蛇舌草中潜在的抗癌化合物。该分析方法被证明是一种快速检测白花蛇舌草潜在抗癌化合物的可行方法。

更新日期：2020-08-20

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