In Vitro Immunotoxicity of Organophosphate Flame Retardants in Human THP-1-Derived Macrophages.,Environmental Science & Technology

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In Vitro Immunotoxicity of Organophosphate Flame Retardants in Human THP-1-Derived Macrophages.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-07-09 , DOI: 10.1021/acs.est.0c01152
Xinyan Li _{1,

2} , Na Li _{1,

2} , Kaifeng Rao ₁ , Qinghui Huang ₃ , Mei Ma _{1,

2}

Affiliation

Scarce attention has been paid to the immunotoxicity of organophosphate flame retardants (PFRs), which poses a challenge to the systematic assessment of their health risks. In this study, a battery of in vitro immunotoxicity screening assays, including adhesion, phagocytosis, and 48 cytokine/chemokine production, was measured after exposing THP-1-derived macrophages to six selected common PFRs (TPHP, TDCPP, TNBP, TOCP, TCEP, and TBOEP) at a noncytotoxic concentration (≤50 μM). Our results showed that TPHP and TBOEP partially attenuated the adhesion and phagocytosis of the THP-1 mφs and that TDCPP caused a functional loss of phagocytosis, implying the potential immunosuppression. In contrast, TNBP and TOCP may cause an immunostimulation by significantly promoting cell adhesion and enhancing phagocytic efficiency. Additionally, the results from a cytokine/chemokine secretion analysis revealed the proinflammatory properties of TDCPP, TPHP, and TBOEP. TOCP was thought to disrupt the inflammatory balance by inhibiting both proinflammatory and antiinflammatory cytokines. TCEP showed no effect on adhesion or phagocytosis and little modulation of cytokine release at this experimental concentration. Overall, this study supports that PFRs can be immunotoxic to macrophages in different ways and provides evidence for developing more sensitive in vitro immunotoxicity bioassay methods.

中文翻译：

有机磷酸酯阻燃剂在人类THP-1衍生的巨噬细胞中的体外免疫毒性。

对有机磷酸酯阻燃剂（PFR）的免疫毒性缺乏关注，这对系统评估其健康风险构成了挑战。在这项研究中，在将THP-1衍生的巨噬细胞暴露于六种选定的常见PFR（TPHP，TDCPP，TNBP，TOCP，TCEP）后，测量了一系列的体外免疫毒性筛选测定法，包括粘附，吞噬作用和48种细胞因子/趋化因子的产生。和TBOEP）以无细胞毒性浓度（≤50μM）。我们的结果表明，TPHP和TBOEP会部分减弱THP-1mφs的粘附和吞噬作用，而TDCPP会导致吞噬作用的功能丧失，暗示潜在的免疫抑制作用。相反，TNBP和TOCP可能通过显着促进细胞粘附并增强吞噬效率而引起免疫刺激。另外，细胞因子/趋化因子分泌分析的结果显示了TDCPP，TPHP和TBOEP的促炎特性。认为TOCP通过抑制促炎和抗炎细胞因子来破坏炎性平衡。在该实验浓度下，TCEP对粘连或吞噬作用没有影响，对细胞因子释放的调节很小。总体而言，这项研究支持PFRs可以以不同方式对巨噬细胞产生免疫毒性，并为开发更敏感的体外免疫毒性生物测定方法提供了证据。在该实验浓度下，TCEP对粘连或吞噬作用没有影响，对细胞因子释放的调节很小。总体而言，这项研究支持PFR可以以不同方式对巨噬细胞产生免疫毒性，并为开发更敏感的体外免疫毒性生物测定方法提供了证据。在该实验浓度下，TCEP对粘连或吞噬作用没有影响，对细胞因子释放的调节很小。总体而言，这项研究支持PFR可以以不同方式对巨噬细胞产生免疫毒性，并为开发更敏感的体外免疫毒性生物测定方法提供了证据。

更新日期：2020-07-21

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