We developed a bidirectional bioelectronic communication system that is enabled by a redox signal transduction modality to exchange information between a living cell-embedded bioelectronics interface and an engineered microbial network. A naturally communicating three-member microbial network is 'plugged into' an external electronic system that interrogates and controls biological function in real time. First, electrode-generated redox molecules are programmed to activate gene expression in an engineered population of electrode-attached bacterial cells. These cells interpret and translate electronic signals and then transmit this information biologically by producing quorum sensing molecules that are, in turn, interpreted by a planktonic co-culture. The propagated molecular communication drives expression and secretion of a therapeutic peptide from one strain and, simultaneously, enables direct electronic feedback from the second strain thus enabling real time electronic verification of biological signal propagation. Overall, we show how this multi-functional bioelectronic platform, termed BioLAN, reliably facilitates on-demand bioelectronic communication and concurrently performs programmed tasks.

中文翻译：

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电子设备与工程多功能微生物群落之间的强大双向通信

我们开发了一种双向生物电子通信系统，该系统通过氧化还原信号转导方式启用，可以在嵌入活细胞的生物电子接口与工程微生物网络之间交换信息。自然沟通的三元微生物网络被“插入”外部电子系统，该系统实时询问和控制生物学功能。首先，对电极产生的氧化还原分子进行编程以激活基因工程化的电极附着细菌细胞群中的基因表达。这些细胞解释并翻译电子信号，然后通过产生群体感应分子，进而通过浮游共培养来解释群体感应分子，从而生物学地传输该信息。传播的分子通讯驱动一个菌株中治疗性肽的表达和分泌，同时使第二个菌株能够进行直接电子反馈，从而实现了生物信号传播的实时电子验证。总体而言，我们展示了称为BioLAN的多功能生物电子平台如何可靠地促进按需生物电子通信并同时执行程序化任务。