3D Bioprinting of Bone Marrow Mesenchymal Stem Cell-Laden Silk Fibroin Double Network Scaffolds for Cartilage Tissue Repair.,Bioconjugate Chemistry

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3D Bioprinting of Bone Marrow Mesenchymal Stem Cell-Laden Silk Fibroin Double Network Scaffolds for Cartilage Tissue Repair.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-07-09 , DOI: 10.1021/acs.bioconjchem.0c00298
Tianyu Ni _{1,

2} , Min Liu ₃ , Yajie Zhang ₁ , Yi Cao ₁ , Renjun Pei ₁

Affiliation

3D bioprinting is one of the latest trends in regenerative medicine due to its capacity for constructing highly organized tissues with living cells. In this work, silk fibroin (SF) together with hydroxypropyl methyl cellulose (HPMC) was used to print bone marrow mesenchymal stem cell (BMSC)-laden double network (DN) hydrogel for cartilage tissue repair. The β-sheet structure formed among SF molecules was set as the rigid and brittle first network, while the cross-linking of HPMC-MA was set as the soft and ductile second network. Compared to the single network hydrogel, the fracture strength, breaking elongation, and compressive reproducibility increased significantly. Thereafter, the evaluation of cell proliferation and biochemical assay of this BMSC-laden 3D bioprinted hydrogel proved that it could ensure sufficient nutrient supply and great biochemical supportability in tissue engineering. This SF-based bioink with remarkable mechanical properties holds great promise as candidate for cartilage tissue regeneration.

中文翻译：

用于软骨组织修复的骨髓间充质干细胞载丝蛋白双网络支架的3D生物打印。

3D生物打印是再生医学的最新趋势之一，因为它具有利用活细胞构建高度组织化的组织的能力。在这项工作中，丝素蛋白（SF）与羟丙基甲基纤维素（HPMC）一起用于打印骨髓间充质干细胞（BMSC）负载的双网（DN）水凝胶，以修复软骨组织。SF分子之间形成的β-折叠结构被设置为刚性和脆性的第一网络，而HPMC-MA的交联被设置为软且可延展的第二网络。与单网络水凝胶相比，断裂强度，断裂伸长率和压缩再现性显着提高。之后，对这种载满BMSC的3D生物打印水凝胶的细胞增殖和生化测定的评估证明，它可以确保组织工程中足够的营养供应和强大的生化支持能力。这种具有优异机械性能的基于SF的生物墨水有望成为软骨组织再生的候选者。

更新日期：2020-08-19

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