Abstract

The study aimed to assess the effect of simvastatin on gene expression of LDLR, SREBPs, and SCD1 in rat hepatic tissues fed with high-fat diets (HFD) and its association with some biochemical parameters. Thirty-two male Wister albino rats were divided into four equal groups (three test and one control groups). The biochemical parameters were determined by using spectrophotometer techniques and the Elisa method. Low-density lipoprotein receptor, sterol regulatory element-binding proteins, stearoyl-CoA desaturase1, Beta-actin were analysed by real-time quantitative polymerase chain reaction (RT-PCR) method. At the end of study, the livers of the rats were separated and changes of hepatic tissue were determined. LDLR, SREBP2, and SCD1 expression increased significantly when compared G1 versus G4 and G2 versus G4. The expression of LDLR, SREBP2, and SCD1 also increased significantly when compared G2 versus G3, G1versus G3 and G1 versus G3 and G2 versus G3. The serum level of cholesterol, triglyceride, glucose, LDL, and HDL increased significantly when compared G1 versus G3. LDL showed significantly decreased when compared G1 versus G2. Cholesterol, glucose and HDL and triglyceride levels were increased significantly when compared G1 versus G4 and G2. Treatment of rats with HFD and simvastatin 20 mg/kg, triglyceride and LDL were almost the same as a control group and LDLR expression increased 98% in liver tissue. Gene expressions may be up-regulated in liver tissue and they showed different effects on biochemical parameters.

摘要

该研究旨在评估辛伐他汀对高脂饮食 (HFD) 喂养的大鼠肝组织中 LDLR、SREBP 和 SCD1 基因表达的影响及其与一些生化参数的关系。将32只雄性Wister白化大鼠分成4个相等的组（3个试验组和1个对照组）。生化参数采用分光光度计技术和Elisa法测定。采用实时定量聚合酶链反应（RT-PCR）方法分析低密度脂蛋白受体、甾醇调节元件结合蛋白、硬脂酰辅酶A去饱和酶1、β-肌动蛋白。研究结束时，分离大鼠肝脏，测定肝组织变化。与 G1 与 G4 以及 G2 与 G4 相比，LDLR、SREBP2 和 SCD1 的表达显着增加。LDLR、SREBP2 的表达，当比较 G2 与 G3、G1 与 G3 和 G1 与 G3 和 G2 与 G3 时，SCD1 也显着增加。与 G1 与 G3 相比，血清胆固醇、甘油三酯、葡萄糖、低密度脂蛋白和高密度脂蛋白水平显着增加。与 G1 与 G2 相比，LDL 显着降低。与 G1 与 G4 和 G2 相比，胆固醇、葡萄糖和 HDL 以及甘油三酯水平显着增加。用HFD和辛伐他汀20 mg/kg治疗大鼠，甘油三酯和LDL几乎与对照组相同，肝组织中LDLR表达增加98%。基因表达可能在肝组织中上调，并且它们对生化参数表现出不同的影响。与 G1 与 G3 相比，HDL 显着增加。与 G1 与 G2 相比，LDL 显着降低。与 G1 与 G4 和 G2 相比，胆固醇、葡萄糖和 HDL 以及甘油三酯水平显着增加。用HFD和辛伐他汀20 mg/kg治疗大鼠，甘油三酯和LDL几乎与对照组相同，肝组织中LDLR表达增加98%。基因表达可能在肝组织中上调，并且它们对生化参数表现出不同的影响。与 G1 与 G3 相比，HDL 显着增加。与 G1 与 G2 相比，LDL 显着降低。与 G1 与 G4 和 G2 相比，胆固醇、葡萄糖和 HDL 以及甘油三酯水平显着增加。用HFD和辛伐他汀20 mg/kg治疗大鼠，甘油三酯和LDL几乎与对照组相同，肝组织中LDLR表达增加98%。基因表达可能在肝组织中上调，并且它们对生化参数表现出不同的影响。甘油三酯和 LDL 与对照组几乎相同，肝组织中 LDLR 表达增加了 98%。基因表达可能在肝组织中上调，并且它们对生化参数表现出不同的影响。甘油三酯和 LDL 与对照组几乎相同，肝组织中 LDLR 表达增加了 98%。基因表达可能在肝组织中上调，并且它们对生化参数表现出不同的影响。