In this study, an eco-friendly renewable biodegradable alternative to petroleum-based oil lubricants was produced using espresso coffee wastes. Waste coffee oil used as raw material was extracted from Espresso coffee wastes by Soxhlet and conventional solvent extraction. Free fatty acids (FFA) were obtained by hydrolysis of the obtained waste oil using Lipozyme TL IM (Thermomyces lanuginosus). The byproduct, glycerol, was also separated from the reaction medium using a separatory funnel. The obtained FFA was used as a raw material in the production of TMP esters. Polyol esters of fatty acids were synthesized as a result of the esterification reaction between FFA and polyol alcohol (trimethylolpropane (TMP)) using Novozyme 435 (Candida antarctica). The amount of FFA in the medium and the FFA conversion was determined by titration with NaOH solution according to ASTM D 5555-95 standard and the FFA composition of espresso coffee oil by GC. The oil content of espresso coffee extract was found to be rich by 16% and the FFA composition was rich in palmitic acid (C_16:0 43% by weight) and linoleic acid (C_18:2 31% by weight). 31% of FFA was obtained from the coffee oil. Experimental studies have shown that the highest FFA conversion of 88% with 93% TMP tri-ester content was obtained at a temperature of 55°C, 5% enzyme (w/w), non-aqueous media, 3/1 FFA/TMP mole ratio, 500 rpm mixing speed and 24 hours.

在这项研究中，使用浓缩咖啡废料生产了一种环保的可生物降解的可替代石油基润滑油的替代品。通过索氏萃取法和常规溶剂萃取法从浓缩咖啡废料中提取了用作原料的废咖啡油。使用Lipozyme TL IM（嗜热霉菌（Thermomyces lanuginosus））通过水解获得的废油获得游离脂肪酸（FFA ）。还使用分液漏斗将副产物甘油与反应介质分离。所得FFA用作TMP酯生产中的原料。通过使用Novozyme 435（南极假丝酵母）FFA与多元醇（三羟甲基丙烷（TMP））之间的酯化反应合成了脂肪酸的多元醇酯）。培养基中的FFA含量和FFA转化率是根据ASTM D 5555-95标准通过用NaOH溶液滴定来确定的，而浓咖啡的FFA组成则是通过GC确定的。发现浓缩咖啡提取物的油含量富含16％，而FFA组合物富含棕榈酸（C _16：0 43重量％）和亚油酸（C _18：2 31重量％）。从咖啡油中获得31％的FFA。实验研究表明，在55°C，5％酶（w / w），非水介质，3/1 FFA / TMP的条件下，可以获得最高的FFA转化率（88％）和93％TMP三酯含量。摩尔比，500 rpm的混合速度和24小时。