Abstract Microbes and their secreted extracellular polymeric substance (EPS) could regulate the mineral phases, morphologies and structure of the microbial induced carbonate precipitates (MICPs). Here, two groups of mineralization experiments were carried out respectively to investigate the mechanism of the microbial induced carbonate precipitation (MICP) using Arthrobacter sp. MF-2 strain and its EPS. X-ray diffraction (XRD) were used to characterize the mineral species and scanning electron microscopy (SEM) was used to investigate the morphologies of the precipitates. Besides, focused ion beam (FIB) was used to prepare calcified bacterial slice for transmission electron microscopy (TEM) analysis. Meanwhile, the temporal change of bacterial density, pH value, conductivity, weight of precipitates, Ca2+ concentration and EPS content were also recorded. The results of this paper showed EPS could serve as the stabilizer of unstable phases and the scaffold for the accumulation of nanoparticles.

中文翻译：

---

微生物诱导碳酸盐沉淀中的纳米颗粒积累：细胞外聚合物物质的关键作用

摘要 微生物及其分泌的胞外聚合物（EPS）可以调节微生物诱导的碳酸盐沉淀物（MICPs）的矿物相、形态和结构。在这里，分别进行了两组矿化实验，以研究使用节杆菌属的微生物诱导碳酸盐沉淀（MICP）的机制。MF-2 菌株及其 EPS。X 射线衍射 (XRD) 用于表征矿物种类，扫描电子显微镜 (SEM) 用于研究沉淀物的形态。此外，聚焦离子束（FIB）用于制备钙化细菌切片用于透射电子显微镜（TEM）分析。同时，细菌密度、pH值、电导率、沉淀物重量、还记录了 Ca2+ 浓度和 EPS 含量。本文的结果表明EPS可以作为不稳定相的稳定剂和纳米颗粒积累的支架。