Abstract Diabetes mellitus is a debilitating health care problem affecting 382 million people around the world and one of the most common complications is diabetic nephropathy. For this reason, it is important to try to identify new mechanisms that could be involved in diabetes. A new class of receptors has been reported, called orphan receptors because the associated ligand and signaling cascades are unknown. These receptors could be an important source of targets for the treatment of many diseases such as diabetes and its associated complications like diabetic nephropathy. Therefore, the aim of this work was to study expression of the orphan receptors GPR149, GPR153, GPR176, TAAR3, TAAR5 and TAAR9 in the kidney of diabetic rats. We used male Wistar rats at 10–12 weeks of age. Diabetes was induced by a single dose of streptozotocin (60 mg/kg i.p.). After 4 weeks, tissues were obtained, and the expression of the mRNAs was measured by RT-PCR. Our results showed that the orphan receptors are expressed in a different way in the kidney. In conclusion, we suggest that orphan receptors could be involved in the development of diabetic nephropathy.

中文翻译：

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糖尿病肾病导致 Wistar 大鼠孤儿受体 GPR149、GPR153、GPR176、TAAR3、TAAR5 和 TAAR9 的组织表达谱发生改变

摘要 糖尿病是影响全球 3.82 亿人的健康问题，最常见的并发症之一是糖尿病肾病。因此，尝试确定可能与糖尿病有关的新机制非常重要。已经报道了一类新的受体，称为孤儿受体，因为相关的配体和信号级联是未知的。这些受体可能是治疗许多疾病（如糖尿病及其相关并发症如糖尿病肾病）的重要靶标来源。因此，本工作的目的是研究孤儿受体 GPR149、GPR153、GPR176、TAAR3、TAAR5 和 TAAR9 在糖尿病大鼠肾脏中的表达。我们使用了 10-12 周龄的雄性 Wistar 大鼠。单剂量链脲佐菌素（60 mg/kg ip ）。4周后，获得组织，并通过RT-PCR测量mRNA的表达。我们的结果表明孤儿受体在肾脏中以不同的方式表达。总之，我们认为孤儿受体可能参与了糖尿病肾病的发展。