ABSTRACT

We investigated the effects of increased intra-abdominal pressure during laparoscopy on the endocrine and exocrine functions, oxidative stress and histopathology of the pancreas in rats. We established three experimental groups of eight animals. Group 1 was the untreated control. Forth other two groups, pneumoperitoneum with CO₂ was established for 60 min at 6 mm Hg for group 2 and 12 mm Hg for group 3; groups 2 and 3 animals were allowed to re-perfuse for 30 min. Amylase, glucagon and insulin levels were analyzed in blood samples and insulin:glucagon ratio was calculated. Histopathology and malondialdehyde assay were performed on pancreatic tissue samples. Histological damage scores for vasocongestion were increased significantly in groups 2 and 3 compared to group 1. Histological damage scores for inflammatory cell infiltration were increased significantly in group 3 compared to group 1. Malondialdehyde levels were increased significantly in group 3 compared to group 1. We found no significant differences among groups for serum amylase levels or histological damage scores for hemorrhage. Insulin and glucagon levels, and the insulin:glucagon ratio was increased significantly in group 3 compared to groups 1 and 2. We found that in rats routine laparoscopy caused increased serum insulin and glucagon levels, and histopathological changes that indicated ischemia-reperfusion injury to the pancreas.

摘要

我们研究了腹腔镜检查期间腹内压升高对大鼠胰腺内分泌和外分泌功能、氧化应激和组织病理学的影响。我们建立了三个实验组，每组八只动物。第 1 组是未处理的对照。第四组，CO ₂气腹在第 2 组 6 mm Hg 和第 3 组 12 mm Hg 下建立 60 分钟；允许第 2 组和第 3 组动物再灌注 30 分钟。分析血液样品中的淀粉酶、胰高血糖素和胰岛素水平并计算胰岛素：胰高血糖素比率。对胰腺组织样品进行组织病理学和丙二醛测定。与第 1 组相比，第 2 组和第 3 组的血管充血组织学损伤评分显着增加。与第 1 组相比，第 3 组炎症细胞浸润的组织学损伤评分显着增加。与第 1 组相比，第 3 组的丙二醛水平显着增加。我们发现各组之间的血清淀粉酶水平或出血的组织学损伤评分没有显着差异。胰岛素和胰高血糖素水平，以及胰岛素：