With the goal of labeling and manipulating the zebrafish hypothalamus, we sought to target a green fluorescent protein (gfp) transgene to the expression domains of nkx2.4b, a gene expressed during hypothalamic and thyroid development. We combined transcription activator‐like effector nucleases (TALENs)‐mediated mutagenesis with a targeting construct to enable insertion of a gfp transgene into the endogenous nkx2.4b genomic locus.

中文翻译：

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针对斑马鱼 nkx2.4b 基因座的转基因驱动特定的绿色荧光蛋白表达并破坏甲状腺发育。

为了标记和操纵斑马鱼下丘脑，我们试图将绿色荧光蛋白 (gfp)转基因靶向 nkx2.4b的表达域，一种在下丘脑和甲状腺发育过程中表达的基因。我们将转录激活因子样效应核酸酶 (TALENs) 介导的诱变与靶向构建体相结合，以将gfp转基因插入内源性nkx2.4b基因组位点。