当前位置：
X-MOL 学术
›
Chem. Biodivers.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Enriched terpenes fractions of Euphorbia umbellata latex promote apoptosis in leukemic cells
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-09-02 , DOI: 10.1002/cbdv.202000369 Luiza Stolz Cruz 1 , Carla Cristine Kanunfre 2 , Evelyn Assis de Andrade 1 , Alexandre Alves de Oliveira 1 , Laura Stolz Cruz 1 , Mariane de Faria Moss 3 , Guilherme Lanzi Sassaki 4 , Leociley Rocha Alencar Menezes 4 , Mei Wang 5 , Ikhlas Ahmad Khan 5 , Flávio Luís Beltrame 1
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-09-02 , DOI: 10.1002/cbdv.202000369 Luiza Stolz Cruz 1 , Carla Cristine Kanunfre 2 , Evelyn Assis de Andrade 1 , Alexandre Alves de Oliveira 1 , Laura Stolz Cruz 1 , Mariane de Faria Moss 3 , Guilherme Lanzi Sassaki 4 , Leociley Rocha Alencar Menezes 4 , Mei Wang 5 , Ikhlas Ahmad Khan 5 , Flávio Luís Beltrame 1
Affiliation
|
The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.
中文翻译：
大戟胶乳中富含萜烯组分促进白血病细胞凋亡
目前的研究是通过生物引导分离伞形大戟乳胶的己烷提取物对抗白血病细胞进行的。通过 NMR、GC/MS、三萜定量和 MTT 还原分析对样品进行分析。对二氯甲烷和乙醇组分进行形态学、细胞周期、线粒体膜电位和半胱天冬酶 3/7 分析，并对二氯甲烷组分进行选择性指数分析。NMR 分析显示了萜烯和类固醇的特征信号，数据通过三萜的定量和 GC/MS 分析得到证实。MTT 还原试验表明 HL-60 是对二氯甲烷和乙醇组分最敏感的细胞谱系。这些基质的化合物引起与细胞凋亡诱导、细胞周期改变、去极化群体细胞的增加和半胱天冬酶的激活 3/7。选择性指数均高于22.44。生物引导分级研究表明，伞形花序的乳胶样品提高了植物复合物对白血病细胞的活性，细胞毒性可能与凋亡途径有关。
更新日期：2020-09-02
中文翻译：
大戟胶乳中富含萜烯组分促进白血病细胞凋亡
目前的研究是通过生物引导分离伞形大戟乳胶的己烷提取物对抗白血病细胞进行的。通过 NMR、GC/MS、三萜定量和 MTT 还原分析对样品进行分析。对二氯甲烷和乙醇组分进行形态学、细胞周期、线粒体膜电位和半胱天冬酶 3/7 分析，并对二氯甲烷组分进行选择性指数分析。NMR 分析显示了萜烯和类固醇的特征信号，数据通过三萜的定量和 GC/MS 分析得到证实。MTT 还原试验表明 HL-60 是对二氯甲烷和乙醇组分最敏感的细胞谱系。这些基质的化合物引起与细胞凋亡诱导、细胞周期改变、去极化群体细胞的增加和半胱天冬酶的激活 3/7。选择性指数均高于22.44。生物引导分级研究表明，伞形花序的乳胶样品提高了植物复合物对白血病细胞的活性，细胞毒性可能与凋亡途径有关。
京公网安备 11010802027423号