The purpose

to study the distribution of Pantoea agglomerans (P. agglomerans) statistically and the presence of bla_PER-1 type ESβL in the clinical and environmental isolates.

Methods

During a period of 2014–2015, 895 blood specimens and 438 hospital environmental samples were collected from one children's hospital in Baghdad city. The results of statistical analysis showed there was no relationship between the infection with P. agglomerans and the sex, while there was a relationship between the infection with the P. agglomerans and the place of residence and also the age of patients.

Result

A total of 23 P. agglomerans were isolated during the study, out of 23 isolates, 13 (56.52%) and 10 (43.48%) were isolated from blood specimens and from hospital environment. All 23 isolates had 100% sensitivity rate to Imipenem and the highest resistant rate was (95.65%) to Ampicillin. Out of 23 P. agglomerans, 14 (60.87%) isolates were positive ESβL producing by the screening test.

Conclusion

The result of molecular screening of the gene bla_PER-1 showed the presence of this gene only in phenotypically ESBL producing isolates, while all negative ESβL producing isolates don't harboring bla_PER-1 gene. Out of 14 positive ESβL producing P. agglomerans isolates, 5 (35.71%) were harboring bla_PER-1 gene and 9 (64.29%) of positive ESβL producing isolates were don't harboring bla_PER-1 gene (significant difference at ≤0.05).

中文翻译：

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泛集聚体分离物的分布和blaPER-1ESβL基因的分子检测：一项统计研究。

目的

以统计学方式研究泛菌（P. agloglomerans）的分布以及临床和环境分离物中bla _PER-1型ESβL的存在。

方法

2014-2015年期间，从巴格达市一家儿童医院收集了895份血液标本和438份医院环境标本。统计分析结果表明，团聚假单胞菌的感染与性别之间没有关系，而团聚假单胞菌的感染与居住地点和患者年龄之间没有关系。

结果

在研究过程中共分离出23种团聚体，从血液样本和医院环境中分离出13株（56.52％）和10株（43.48％）。所有23株菌株对亚胺培南的敏感性均为100％，对氨苄西林的最高耐药率为（95.65％）。通过筛选测试，在23个团聚毕生球菌中，有14个（60.87％）分离物为阳性ESβL。

结论

bla _PER-1基因的分子筛查结果表明，该基因仅在表型上产生ESBL的分离株中存在，而所有阴性ESβL产生的分离株都没有bla _PER-1基因。在14株产生ESβL的阳性附球菌中，有5株（35.71％）带有bla _PER-1基因，有9株（64.29％）产生ESβL的阳性菌株中没有bla _PER-1基因（显着性差异≤0.05 ）。