The current study examined the cell adherence property of probiotic Pediococcus pentosaceus GS4 (MTCC12683) with the characterization and functionality in adherence of its surface layer protein (GS4-Slp). The Slp of P. pentosaceus GS4 was extracted purified and detected using SDS-PAGE (98 kDa) and size exclusion chromatography. The cell adherence property of probiotic GS4 (Slp⁺/Slp⁻) was evaluated on buccal cells and HCT-116. Purified Slp was found neutralized with raised anti-Slp showing reduced adherence to HCT-116 as evident from SEM analysis. The structure of GS4-Slp was determined by MALDI-TOF analysis, CD analysis, atomic force microscopy (AFM), and FT-IR spectrometry. In Silico approach revealed its indirect similarity with cell membrane protein of Helicobacter pylori. Results thus reveal that GS4 has the potential of the production of 98 kDa Slp which facilitates the cell adherence property. This added probiotic attribute will enhance the probiotic potentials of P. pentosaceus GS4 to use it biotechnologically.

Significance

Probiotic Pediococcus pentosaceus GS4 facilitates demonstrable colonization by the elaboration of Slp. This property imparts a value to the strain and claims to be more useful biotechnologically.

中文翻译：

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益生菌戊糖片球菌GS4（MTCC 12683）的细胞粘附功效及其表层蛋白（Slp）的作用可证明。

当前的研究检查了益生菌戊糖片球菌GS4（MTCC12683）的细胞粘附特性，以及其表层蛋白（GS4-Slp）的粘附特性和功能。提取纯化的戊糖假单胞菌GS4的Slp，并使用SDS-PAGE（98 kDa）和尺寸排阻色谱法进行检测。益生菌GS4（SLP的细胞粘附特性⁺ / SLP ^- ）对口腔细胞和HCT-116进行了评价。从SEM分析中可以看出，纯化的Slp被升高的抗Slp所中和，显示出对HCT-116的粘附性降低。GS4-Slp的结构是通过MALDI-TOF分析，CD分析，原子力显微镜（AFM）和FT-IR光谱测定的。在硅该方法揭示了它与幽门螺杆菌细胞膜蛋白的间接相似性。结果因此表明GS4具有产生98kDa Slp的潜力，其促进了细胞粘附特性。这种增加的益生菌特性将增强戊糖假单胞菌GS4在生物技术上使用它的益生菌潜力。

意义

益生菌Petococcus pentosaceus GS4通过精制Slp促进了明显的定殖。该性质赋予菌株以价值，并声称在生物技术上更有用。