Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-07-09 , DOI: 10.1016/j.jprot.2020.103895 Nastaran Mehri 1 , Reza Fotovat 1 , Mehdi Mirzaei 2 , Ehsan Mohseni Fard 1 , Pouria Parsamatin 3 , Mafruha T Hasan 4 , Yunqi Wu 5 , Mohammad Reza Ghaffari 6 , Ghasem Hosseini Salekdeh 7
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Reproductive stage is very sensitive to various forms of environmental stresses such as drought stress. The proteomic analysis of anther during pollen development in response to drought stress was performed using a label-free quantitative shotgun proteomic technique to define the underlying molecular principles in two contrasting wheat genotypes Shiraz (susceptible) and D-10 (tolerant). Drought stress resulted in around two-fold decline in seed setting capacity and pollen viability in the Shiraz genotype compared to D-10. A Partial Least Square Discriminant Analysis (PLS-DA) of proteomic data revealed the abundance of 131 differentially abundant proteins significantly contributing in separation of drought tolerant and susceptible genotypes under normal and stress conditions. Proteins involved in cellular respiration, carbohydrate metabolism, RNA metabolism, and vesicle trafficking showed completely different responses in two genotypes. These proteins may maintain hexose pool and energy level and control regulation of transcription and transport. Furthermore, different members of functional groups such as protein biosynthesis and degradation, chromatin organization, and cytoskeleton dynamics were differentially abundant in response to stress in both genotypes which suggest their function in both genotypes to maintain minimum pollen viability/ fertility under drought stress. In conclusion, our findings revealed various metabolic and regulatory pathways underlying survival strategies required for pollen fertility and viability.
Significance
Drought caused by global climate change decreases cereal grain productivity worldwide. Yield losses due to water stress have been reported for major small grain cereal including wheat. Our findings highlighted the importance of key proteins in wheat adaptation to drought stress at reproductive stage. The obtained data showed that differentially abundant proteins in drought tolerant wheat genotype was remarkably associated with cellular respiration, carbohydrate metabolism, RNA metabolism, and vesicle trafficking. These results revealed fundamental data to elucidate the complexity of pollen fertility and viability under drought stress condition in wheat.
中文翻译：
小麦不同基因型的蛋白质组学分析揭示了干旱胁迫下花药中主要代谢途径和调节途径之间的相互作用。
生殖阶段对各种形式的环境胁迫（如干旱胁迫）非常敏感。使用无标记的定量analysis弹枪蛋白质组学技术对花粉发育过程中的花药进行蛋白质组学分析，以确定花粉发育过程中干旱胁迫的响应，该分子组技术定义了两种不同的小麦基因型设拉子（易感）和D-10（耐性）。与D-10相比，干旱胁迫导致设拉子基因型的结实能力和花粉生存力下降了大约两倍。蛋白质组学数据的偏最小二乘判别分析（PLS-DA）显示，存在131种差异丰富的蛋白质，这些蛋白质在正常和胁迫条件下显着有助于分离耐旱和易感基因型。参与细胞呼吸，碳水化合物代谢，RNA代谢和囊泡运输显示两种基因型完全不同的反应。这些蛋白质可以维持己糖池和能量水平，并控制转录和转运的调节。此外，两种基因型对蛋白质胁迫的响应均不同，例如蛋白质的生物合成和降解，染色质组织和细胞骨架动力学等功能基团的丰富程度不同，这表明它们在两种基因型中的功能均能在干旱胁迫下维持最低的花粉生存力/繁殖力。总之，我们的发现揭示了花粉育性和生存力所必需的生存策略的各种代谢和调节途径。这些蛋白质可以维持己糖池和能量水平，并控制转录和转运的调节。此外，两种基因型对蛋白质胁迫的响应均不同，例如蛋白质的生物合成和降解，染色质组织和细胞骨架动力学等功能基团的丰富程度不同，这表明它们在两种基因型中的功能均能在干旱胁迫下维持最低的花粉生存力/繁殖力。总之，我们的发现揭示了花粉育性和生存力所必需的生存策略的各种代谢和调节途径。这些蛋白质可以维持己糖池和能量水平，并控制转录和转运的调节。此外，两种基因型对蛋白质胁迫的响应均不同，例如蛋白质的生物合成和降解，染色质组织和细胞骨架动力学等功能基团的丰富程度不同，这表明它们在两种基因型中的功能均能在干旱胁迫下维持最低的花粉生存力/繁殖力。总之，我们的发现揭示了花粉育性和生存力所必需的生存策略的各种代谢和调节途径。两种基因型在胁迫下的细胞骨架和细胞动力学均差异丰富，这表明它们在两种基因型下的功能在干旱胁迫下均能维持最小的花粉生存力/繁殖力。总之，我们的发现揭示了花粉育性和生存力所必需的生存策略的各种代谢和调节途径。两种基因型在胁迫下的细胞骨架和细胞动力学均差异丰富，这表明它们在两种基因型下的功能在干旱胁迫下均能维持最小的花粉生存力/繁殖力。总之，我们的发现揭示了花粉育性和生存力所必需的生存策略的各种代谢和调节途径。
意义
全球气候变化引起的干旱降低了全世界谷物的生产力。据报道，包括小麦在内的主要小谷类谷物因水分胁迫而损失了产量。我们的发现强调了关键蛋白在小麦适应生殖阶段干旱胁迫中的重要性。获得的数据表明，在耐旱小麦基因型中差异丰富的蛋白质与细胞呼吸，碳水化合物代谢，RNA代谢和囊泡运输显着相关。这些结果揭示了基础数据，阐明了干旱胁迫条件下小麦花粉育性和花粉活力的复杂性。
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