Annona muricata L. is used in folk medicine for treatment of diseases related to inflammatory and oxidative processes. This study investigated the effect of the aqueous extract of A. muricata leaves (AEAM) on TPA-induced ear inflammation and antioxidant capacity, both in vitro and in vivo. The in vitro antioxidant capacity of AEAM was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP) and lipoperoxidation assays. Cytotoxicity and reactive oxygen species (ROS) release were evaluated in the L929 fibroblasts. Swiss mice were submitted to TPA application and were topically treated with AEAM (0.3, 1 or 3 mg/ear). After 6 h, inflammatory and oxidative parameters were evaluated. Quercetin 3-glucoside, rutin, chlorogenic acid, catechin and gallic acid were identified in AEAM. It also presented antioxidant activity in all in vitro assays used. Incubation with AEAM did not cause cell cytotoxicity but reduced ROS release from fibroblasts. Compared with the control group, treatment with AEAM significantly reduced ear oedema and mieloperoxidase activity in inflamed ears, as well as histological parameters of inflammation. These results were associated with the reduction of total hydroperoxides and modulation of catalase, but not superoxide dismutase activity. These findings show the anti-inflammatory effect of AEAM is associated with antioxidant capacity.

番荔枝在民间医学中用于治疗与炎症和氧化过程相关的疾病。本研究调查了A. muricata水提取物的影响叶 (AEAM) 在体外和体内对 TPA 诱导的耳部炎症和抗氧化能力的影响。AEAM 的体外抗氧化能力是通过 2,2-diphenyl-1-picrylhydrazyl (DPPH)、铁还原/抗氧化能力 (FRAP) 和脂质过氧化试验来测量的。在 L929 成纤维细胞中评估了细胞毒性和活性氧 (ROS) 释放。瑞士小鼠接受 TPA 应用，并用 AEAM（0.3、1 或 3 毫克/耳）进行局部治疗。6 小时后，评估炎症和氧化参数。在 AEAM 中鉴定了槲皮素 3-葡萄糖苷、芦丁、绿原酸、儿茶素和没食子酸。它还在所有使用的体外测定中显示了抗氧化活性。与 AEAM 孵育不会引起细胞毒性，但会减少成纤维细胞的 ROS 释放。与对照组相比，AEAM 治疗显着降低了发炎耳朵的耳水肿和微过氧化物酶活性，以及​​炎症的组织学参数。这些结果与总氢过氧化物的减少和过氧化氢酶的调节有关，但与超氧化物歧化酶活性无关。这些发现表明 AEAM 的抗炎作用与抗氧化能力有关。