Background Gibberellic acid (GA3) is a plant growth regulator used to improve the quality of crops but its residues in food causes many hazardous effects. In contrast, olive oil has registered several health benefits including antioxidant, anti-inflammatory, and anti-cancer. Thus, the present study suggests the use of olive oil as a natural food source to counteract the GA3 toxicity during mice development. In a preliminary experiment, 18 mature females were classified into control and GA3-treated subgroups with ascending doses of GA3 (55, 110, 240, 480, 960 mg/kg B.W.) for 2 weeks. In the main experiment, 20 pregnant females at the 7th day of gestation were divided into four groups: G1 is control, G2 treated orally with GA3 (55 mg/kg), G3 treated with olive oil, and G4 treated with GA3-olive oil. The pregnant females were dissected at prenatal stages at E14 and E18 of gestation. Results The high doses of GA3 in the preliminary experiment showed decrease of uterine folds, reduction of carbohydrates content and TNFR2 expression of the uterine glands, degeneration of the ovarian follicles, blood vessels congestion, and altered TNFR2 expression in oocyte membrane as compared with the control. In the second experiment, GA3-treated embryo at E14 and E18 revealed histopathological changes and altered TNFR2 immunostaining in the developing liver, kidney, and skin tissues. Treatment of GA3 with olive oil improves the negative effects induced by GA3. Conclusion The study concluded that a supplementation rich diet with olive oil creates a protective effect against gibberellic acid-induced embryotoxicity during pregnancy.

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橄榄油对赤霉酸诱导的小鼠产前胚胎毒性的保护作用

背景 赤霉酸 (GA3) 是一种植物生长调节剂，用于改善作物质量，但其在食品中的残留会造成许多危害。相比之下，橄榄油具有多种健康益处，包括抗氧化、抗炎和抗癌。因此，本研究建议使用橄榄油作为天然食物来源来抵消小鼠发育过程中的 GA3 毒性。在初步实验中，将 18 只成熟雌性分为对照组和 GA3 治疗亚组，其中 GA3 剂量递增（55、110、240、480、960 毫克/千克体重），持续 2 周。主要实验中，20只妊娠第7天的怀孕女性被分为4组：G1为对照组，G2口服GA3（55mg/kg），G3橄榄油治疗，G4GA3-橄榄油治疗. 在妊娠 E14 和 E18 的产前阶段解剖怀孕的雌性。结果初步实验中高剂量GA3与对照组相比，子宫皱襞减少，碳水化合物含量减少，子宫腺体TNFR2表达减少，卵泡退化，血管充血，卵母细胞膜TNFR2表达改变。 . 在第二个实验中，在 E14 和 E18 的 GA3 处理胚胎揭示了发育中的肝脏、肾脏和皮肤组织中的组织病理学变化和 TNFR2 免疫染色的改变。用橄榄油处理 GA3 可改善 GA3 引起的负面影响。结论 该研究得出结论，富含橄榄油的膳食补充剂对妊娠期间赤霉酸诱导的胚胎毒性具有保护作用。