Heat and drought are serious threats for crop growth and development. As the sixth largest cereal crop in the world, pearl millet can not only be used for food and forage but also as a source of bioenergy. Pearl millet is highly tolerant to heat and drought. Given this, it is considered an ideal crop to study plant stress tolerance and can be used to identify heat-resistant genes. In this study, we used Pacbio sequencing data as a reference sequence to analyze the Illumina data of pearl millet that had been subjected to heat and drought stress for 48 h. By summarizing previous studies, we found 26,299 new genes and 63,090 new transcripts, and the number of gene annotations increased by 20.18%. We identified 2792 transcription factors and 1223 transcriptional regulators. There were 318 TFs and 149 TRs differentially expressed under heat stress, and 315 TFs and 128 TRs were differentially expressed under drought stress. We used RNA sequencing to identify 6920 genes and 6484 genes differentially expressed under heat stress and drought stress, respectively. Through Pacbio sequencing, we have identified more new genes and new transcripts. On the other hand, comparing the differentially expressed genes under heat tolerance with the DEGs under drought stress, we found that even in the same pathway, pearl millet responds with a different protein.

中文翻译：

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基于Pacbio全长转录组测序的珍珠小米热胁迫和干旱胁迫的转录组分析。

高温和干旱是对作物生长和发育的严重威胁。作为世界第六大谷类作物，珍珠粟不仅可以用作粮食和饲料，还可以用作生物能源。珍珠粟极耐高温和干旱。鉴于此，它被认为是研究植物抗逆性的理想作物，可用于鉴定耐热基因。在这项研究中，我们使用Pacbio测序数据作为参考序列来分析遭受热和干旱胁迫48小时的珍珠粟的Illumina数据。通过总结以前的研究，我们发现了26,299个新基因和63,090个新转录本，基因注释的数量增加了20.18％。我们确定了2792个转录因子和1223个转录调节因子。在热胁迫下有318个TF和149个TR差异表达，在干旱胁迫下差异表达了315个TF和128个TR。我们使用RNA测序来鉴定分别在热胁迫和干旱胁迫下差异表达的6920个基因和6484个基因。通过Pacbio测序，我们发现了更多新基因和新转录本。另一方面，将耐热性下的差异表达基因与干旱胁迫下的DEGs进行比较，我们发现，即使在相同的途径中，珍珠小米也能响应不同的蛋白质。