Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts. Genomic DNA was extracted from 24 paired epidermal samples of warts and normal skin. DNA samples were bisulfite converted and underwent genome-wide methylation profiling using the Infinium MethylationEPIC BeadChip Kit. From a total of 844,234 CpG sites, 56,960 and 43,040 CpG sites were found to be hypo- and hypermethylated, respectively, in non-genital cutaneous warts. The most differentially methylated CpG sites in warts were located within the C10orf26, FAM83H-AS1, ZNF644, LINC00702, GSAP, STAT5A, HDAC4, NCALD, and EXOC4 genes. Non-genital cutaneous warts exhibit a unique CpG methylation signature.

中文翻译：

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非生殖器皮肤疣甲基化 CpG 位点的全基因组鉴定。


低危 HPV 感染尚未成为表观遗传学研究的主题。本研究的目的是调查非生殖器皮肤疣中 CpG 位点的甲基化状态。从 24 对疣和正常皮肤的表皮样本中提取基因组 DNA。 DNA 样品经过亚硫酸氢盐转化，并使用 Infinium MmethylationEPIC BeadChip 试剂盒进行全基因组甲基化分析。在非生殖器皮肤疣中，总共 844,234 个 CpG 位点中，分别发现 56,960 个和 43,040 个 CpG 位点低甲基化和高甲基化。疣中差异最大的 CpG 位点位于 C10orf26、FAM83H-AS1、ZNF644、LINC00702、GSAP、STAT5A、HDAC4、NCALD 和 EXOC4 基因内。非生殖器皮肤疣表现出独特的 CpG 甲基化特征。