Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.,Food & Function

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Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
Food & Function ( IF 5.1 ) Pub Date : 2020-07-08 , DOI: 10.1039/c9fo02772f
Ruichang Gao ₁ , Yang Shen , Wanghui Shu , Wengang Jin , Fan Bai , Jinlin Wang , Yuhao Zhang , Hesham El-Seedi , Quancai Sun , Li Yuan

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Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste. Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities. Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon. The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms. The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores. SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α. Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon. Furthermore, SH partially restored the alteration of the gut microbiota in colitic mice. SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae. These results suggest that SH inhibited DSS-induced colitis by regulating the NF-κB and MAPK pathways and modulating microbiota composition.

中文翻译：

urge鱼可通过调节NF-κB，MAPK和微生物群组成来水解减轻DSS诱导的结肠炎。

鱼子酱生产后收集的鱼肌肉副产物通常没有得到充分利用，有时可能会被丢弃，从而造成大量浪费。然而，据报道，可能源自ates鱼肌肉的饮食蛋白水解产物具有多种有益的生物活性。对st鱼肌肉水解产物的生物学活性进行研究，有望为st鱼增加价值。当前的研究旨在研究of鱼肌肉衍生的水解产物的治疗性抗炎作用及其潜在机制。body鱼水解物（SH）的使用显着降低了DSS诱导的损害的严重程度，这通过增加体重，结肠长度以及降低疾病活动指数（DAI）和组织学评分来证明。SH还抑制髓过氧化物酶（MPO）活性并降低血清IL-6，IL-1β和TNF-α的水平。蛋白质印迹结果表明，SH抑制了DSS诱导的结肠中NF-κB和MAPK途径的激活。此外，SH部分恢复了结肠炎小鼠的肠道菌群的变化。SH增加了拟杆菌/拟杆菌比和Ruminococcaceae，Porphyromonadaceae和Bacteroidetes S24-7的相对丰度，同时降低了潜在有害细菌Erysipelotrichaceaceae和Enterocococcaceae的丰度。这些结果表明SH通过调节NF-κB和MAPK途径并调节微生物群组成来抑制DSS诱导的结肠炎。蛋白质印迹结果表明，SH抑制了DSS诱导的结肠中NF-κB和MAPK途径的激活。此外，SH部分恢复了结肠炎小鼠中肠道菌群的改变。SH增加了拟杆菌/拟杆菌比和Ruminococcaceae，Porphyromonadaceae和Bacteroidetes S24-7的相对丰度，同时降低了潜在有害细菌Erysipelotrichaceaceae和Enterocococcaceae的丰度。这些结果表明SH通过调节NF-κB和MAPK途径并调节微生物群组成来抑制DSS诱导的结肠炎。蛋白质印迹结果表明，SH抑制了DSS诱导的结肠中NF-κB和MAPK途径的激活。此外，SH部分恢复了结肠炎小鼠的肠道菌群的变化。SH增加了拟杆菌/拟杆菌比和Ruminococcaceae，Porphyromonadaceae和Bacteroidetes S24-7的相对丰度，同时降低了潜在有害细菌Erysipelotrichaceaceae和Enterocococcaceae的丰度。这些结果表明SH通过调节NF-κB和MAPK途径并调节微生物群组成来抑制DSS诱导的结肠炎。同时减少了潜在有害细菌丹参和肠球菌的丰度。这些结果表明SH通过调节NF-κB和MAPK途径并调节微生物群组成来抑制DSS诱导的结肠炎。同时减少了潜在有害细菌丹参和肠球菌的丰度。这些结果表明SH通过调节NF-κB和MAPK途径并调节微生物群组成来抑制DSS诱导的结肠炎。

更新日期：2020-08-19

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