Simultaneous detection of small molecules, proteins and microRNAs using single molecule arrays,Chemical Science

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Simultaneous detection of small molecules, proteins and microRNAs using single molecule arrays
Chemical Science ( IF 7.6 ) Pub Date : 2020-07-08 , DOI: 10.1039/d0sc02552f
Xu Wang _{1,

2} , David R Walt _{1,

2}

Affiliation

Biological samples such as blood, urine, cerebrospinal fluid and saliva contain a large variety of proteins, nucleic acids, and small molecules. These molecules can serve as potential biomarkers of disease and therefore, it is desirable to simultaneously detect multiple biomarkers in one sample. Current detection techniques suffer from various limitations including low analytical sensitivity and complex sample processing. In this work, we present an ultrasensitive method for simultaneous detection of small molecules, proteins and microRNAs using single molecule arrays (Simoa). Dye-encoded beads modified with specific capture probes were used to quantify each analyte. Multiplex competitive Simoa assays were established for simultaneous detection of cortisol and prostaglandin E2. In addition, competitive and sandwich immunoassays were combined with a direct nucleic acid hybridization assay for simultaneous detection of cortisol, interleukin 6 and microRNA 141. The multi-analyte Simoa assay shows high sensitivity and specificity, which provides a powerful tool for the analysis of many different samples.

中文翻译：

使用单分子阵列同时检测小分子、蛋白质和 microRNA

血液、尿液、脑脊液和唾液等生物样品含有多种蛋白质、核酸和小分子。这些分子可以作为疾病的潜在生物标志物，因此，需要同时检测一个样品中的多种生物标志物。当前的检测技术受到各种限制，包括分析灵敏度低和样品处理复杂。在这项工作中，我们提出了一种使用单分子阵列 (Simoa) 同时检测小分子、蛋白质和 microRNA 的超灵敏方法。用特定捕获探针修饰的染料编码珠用于量化每种分析物。建立了多重竞争性 Simoa 测定法，用于同时检测皮质醇和前列腺素 E2。此外，竞争性和夹心免疫分析与直接核酸杂交分析相结合，可同时检测皮质醇、白细胞介素 6 和 microRNA 141。多分析物 Simoa 分析显示出高灵敏度和特异性，为多种分析提供了强大的工具。不同的样品。

更新日期：2020-08-05

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