Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention.

中文翻译：

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石shan霍乱石end多糖对高胆固醇饮食诱导的斑马鱼动脉粥样硬化的保护作用。

心血管疾病是最高的死亡原因，而动脉粥样硬化（AS）是许多心血管疾病的主要发病机理。在本研究中，我们旨在研究霍山石end的可能药物作用。AS中的CZ Tang等人的SJ Cheng多糖（DHP）。我们用高胆固醇饮食（HCD）喂养斑马鱼，以建立斑马鱼AS模型并用DHP处理，并在荧光显微镜下观察斑块形成和中性粒细胞计数。接下来，利用平行流室建立低剪切应力-（LSS-）诱导的内皮细胞（EC）功能障碍模型。我们观察到DHP显着改善了HCD诱导的脂质沉积，氧化应激和炎症反应，主要表明DHP显着提高了超氧化物歧化酶（SOD）活性，减少了斑块形成，并减少了中性粒细胞募集和总胆固醇（TC）的水平，甘油三酸酯（TG），丙二醛（MDA）和活性氧（ROS）。此外，DHP显着改善了LSS诱导的氧化应激和EC功能障碍。我们的结果表明，DHP可以对AS发挥治疗作用，这可能归因于DHP的降血脂，抗氧化剂，抗炎活性以及改善LSS诱导的EC功能障碍。作为针对AS预防的功能性天然药物来源，DHP具有广阔的发展潜力。