Gastric cancer is one of the most common malignancies worldwide, with high morbidity and poor survival rate. Its prognosis remains unsatisfactory, with a 5-year survival rate of <30%. Studies have indicated that Huaier granules have good antitumor efficacy and safety in several solid malignant tumors. Recent studies have also found that Huaier polysaccharides can promote apoptosis in numerous tumor cells, although only few studies have focused on the effects of Huaier granules on gastric cancers and the mechanisms underlying their antitumor role. We retrospectively evaluated stage IIb gastric cancer patients at Xiangya Hospital, Central South University, through our outpatient system from January 2013 to December 2015. Fifty-four patients were in the Huaier+Tegafur Gimeracil Oteracil Potassium (TGOP) group and 72 in the TGOP group. Further, we conducted CCK8, colony formation, Annexin V-FITC/PI, Western blot, RT-PCR, and plasmid transfection assays to analyze the mechanism by which Huaier polysaccharides play an antitumor role. We confirmed that Huaier granules combined with Tegafur Gimeracil Oteracil Potassium could promote patient prognosis, with a better disease-free survival rate ( vs. , ) and overall survival rate ( vs. , ). Moreover, through cell proliferation assays, Western blot, RT-PCR, and detection of Livin expression at the mRNA and protein levels, we found that Huaier polysaccharides could promote gastric cancer cell apoptosis and inhibit gastric cancer cell proliferation in a time- and dose-dependent manner. Finally, we demonstrated that Huaier polysaccharides promote gastric cancer cell apoptosis through the regulation of Livin expression. Overexpression of Livin reversed the gastric cell apoptosis induced by Huaier polysaccharides. Huaier granules combined with Tegafur Gimeracil Oteracil Potassium ameliorated stage IIb gastric cancer prognosis and induced gastric cancer cell apoptosis by regulating Livin.

中文翻译：

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怀尔颗粒与替加福吉美拉西尔奥曲西钾联用可通过调节Livin促进IIb期胃癌预后并诱导胃癌细胞凋亡。

胃癌是全世界最常见的恶性肿瘤之一，发病率高，生存率低。其预后仍然不能令人满意，其5年生存率<30％。研究表明，怀耳颗粒剂在几种实体恶性肿瘤中具有良好的抗肿瘤功效和安全性。最近的研究还发现，尽管只有很少的研究集中在怀尔颗粒对胃癌的作用及其潜在的抗肿瘤作用机理上，但怀尔多糖可以促进许多肿瘤细胞的凋亡。我们回顾性评估了2013年1月至2015年12月在中南大学湘雅医院通过门诊系统进行的IIb期胃癌患者。其中，有54名患者为怀尔+替加氟吉美拉西奥汀钾（TGOP）组，其中72名为TGOP组。进一步，我们进行了CCK8，集落形成，膜联蛋白V-FITC / PI，蛋白质印迹，RT-PCR和质粒转染实验，以分析槐耳多糖发挥抗肿瘤作用的机制。我们证实，怀耳颗粒联合替加福吉美拉西尔奥特卡西钾能促进患者的预后，无病生存率更高（ 与 ， ）和总体生存率（ 与 ， ）。此外，通过细胞增殖测定，Western印迹，RT-PCR和在mRNA和蛋白质水平检测Livin表达，我们发现怀尔多糖可以在一定的时间和剂量下促进胃癌细胞凋亡并抑制胃癌细胞的增殖。依赖方式。最后，我们证明了槐耳多糖通过调节Livin表达促进胃癌细胞凋亡。Livin的过度表达逆转了槐耳多糖诱导的胃细胞凋亡。怀尔颗粒联合替加福吉美拉西酯/奥特那西钾可通过调节Livin改善IIb期胃癌的预后并诱导胃癌细胞凋亡。