Dendrobium officinale caulis is the dried mature stems (Mat) of D. officinale Kimura et Migo. D. officinale protocorms (DOPs) are the conical embryonic tissues formed after seed germination. Previous studies suggest that these different plant parts possess similar pharmacological activities. However, the metabolic profiles and gene expression across different growth stages of D. officinale remain unknown. In this study, metabolome analysis based on GC and LC/MS/MS was carried out to assess the metabolic profile of both DOPs and Mat. Transcriptome analysis was used to reveal internal relationships between metabolites and the differing gene regulation. The results indicated that although DOPs and Mat had a similar chemical composition, they exhibited different patterns of content. GO classification and KEGG clustering of the differentially expressed genes (DEGs) between DOPs and Mat demonstrated that gene enrichment was mainly related to flavonoid biosynthetic and metabolic processes, carbohydrate metabolic processes, and polysaccharide catabolic processes. An alteration in gene expression related to specific metabolic pathways offered a probable explanation for the observed differences in metabolites between DOPs and Mat. This work provides a foundation for subsequent studies regarding the biosynthetic pathways of related metabolites from D. officinalis.

石铁皮石uli是D. officinale Kimura et Migo的干燥成熟茎（Mat）。D. officinale protocorms（DOPs）是种子发芽后形成的圆锥形胚组织。先前的研究表明这些不同的植物部位具有相似的药理活性。然而，代谢谱和整个的不同生长阶段的基因表达D.石斛仍然未知。在这项研究中，进行了基于GC和LC / MS / MS的代谢组分析，以评估DOP和Mat的代谢特征。转录组分析用于揭示代谢物和不同基因调控之间的内部关系。结果表明，尽管DOPs和Mat的化学成分相似，但它们显示出不同的含量模式。GO分类和DOP和Mat之间的差异表达基因（DEG）的KEGG聚类表明，基因富集主要与类黄酮的生物合成和代谢过程，碳水化合物代谢过程以及多糖分解代谢过程有关。与特定代谢途径相关的基因表达改变为观察到的DOPs和Mat之间的代谢物差异提供了可能的解释。D.厚朴。