Abstract

Drought seriously threatens tomato production worldwide. Despite much research on exogenous salicylic acid (SA) and Ca²⁺ improving plant resistance to biotic and abiotic stress, the molecular mechanisms of exogenous SA- and Ca²⁺-mediated drought resistance response in tomato remain unclear. In this study, we analyzed SA- and Ca²⁺ -induced differentially expressed transcripts under drought in tomato plants using cDNA-amplified fragment length polymorphism (cDNA-AFLP). In total, 34 transcript derived fragments (TDFs) were differentially expressed. The functions identified through NCBI BLAST alignment mainly involved signal transduction, amino acid metabolism, transcription factors, transfer transport and stress response. The quantitative real-time polymerase chain reaction results of 12 TDFs associated with drought response were consistent with the patterns of changes observed with cDNA-AFLP analysis. These differentially expressed transcripts may be used for functional verification, transgenic research and breeding of drought-resistant tomato varieties.

摘要

干旱严重威胁着全世界的番茄生产。尽管对外源水杨酸（SA）和Ca ²⁺改善植物对生物和非生物胁迫的抗性进行了大量研究，但是外源SA和Ca ²⁺介导的番茄抗旱反应的分子机制仍不清楚。在这项研究中，我们分析了SA-和Ca ²⁺cDNA扩增片段长度多态性（cDNA-AFLP）技术在干旱条件下诱导番茄植株的差异表达转录本。总共有34个转录物衍生片段（TDF）差异表达。通过NCBI BLAST比对鉴定的功能主要涉及信号转导，氨基酸代谢，转录因子，转移转运和应激反应。与干旱响应相关的12种TDF的实时定量实时聚合酶链反应结果与通过cDNA-AFLP分析观察到的变化模式一致。这些差异表达的转录本可用于功能验证，转基因研究和抗旱番茄品种的育种。