In our study, the anti-quorum sensing (QS) activity of fermentation broth from TRM B-02, a bacterium isolated from Taklimakan desert, was investigated using the biosensor bioassay on Chromobacterium violaceum ATCC12472. TRM B-02 was 100% similar to Bacillus subtilis subsp. Inaquosorum KCTC 13429^(T) by genotypic and phenotypic analyses. Based on anti-QS activity tracking, six known amicoumacins, named as AI-77-H (1), AI-77-F (2), amicoumacin B (3), amicoumacin C (4), AI-77-C (5) and bacilosarcins D (6), were isolated and identified. Among them, compounds 1–3 exhibited a better inhibitory effect on C. violaceum ATCC12472. Further research suggested that compounds 1–3 could significantly down-regulate the expressions of violacein operon A (vioA), vioB, vioD and vioE and up-regulate vioC. Docking experiments indicated that compounds 1–3 may act as an inhibitor of violacein biosynthetic pathway competitively inhibiting the binding of flavin adenine dinucleotide (FAD) with the vioD enzyme.

在我们的研究中，使用生物传感器生物测定法对紫罗兰色杆菌ATCC12472进行了研究，研究了从塔克拉玛干沙漠中分离出的细菌TRM B-02的发酵液的抗群体感应（QS）活性。TRM B-02与枯草芽孢杆菌亚种100％相似。Inaquosorum KCTC 13429 ^（T）的基因型和表型分析。基于抗QS活性跟踪，六种已知的烟碱霉素，分别称为AI-77-H（1），AI-77-F（2），烟碱霉素B（3），烟碱霉素C（4），AI-77-C（5）和细菌素D（6），被分离和鉴定。其中，化合物1-3显示对一个较好的抑制作用C.堇色ATCC12472。进一步的研究表明，化合物1 - 3可以显著下调紫色杆菌素的表达操纵子A（vioA），vioB，vioD和vioE和上调VIOC。对接实验表明，化合物1-3可以作为紫胶素生物合成途径的抑制剂，竞争性地抑制黄素腺嘌呤二核苷酸（FAD）与vioD酶的结合。