Sin Nombre virus (SNV) is the major cause of hantavirus cardiopulmonary syndrome (HCPS) in North America, a severe respiratory disease with a high fatality rate. SNV is carried by Peromyscus maniculatus, or deer mice, and human infection occurs following inhalation of aerosolized virus in mouse excreta or secreta, often in peri-domestic settings. Currently there are no FDA approved vaccines or therapeutics for SNV or any other hantaviruses, therefore prevention of infection is an important means of reducing the disease burden of HCPS. One approach for preventing HCPS cases is to prevent the spread of the virus amongst the rodent reservoir population through bait vaccination. However, bait style vaccines for rodent-borne viruses have not been employed in the field, unlike those targeting larger species. Here we utilized a recombinant vesicular stomatitis virus expressing SNV glycoprotein precursor (rVSVΔG/SNVGPC) in an attempt to prevent SNV transmission. Vaccination of deer mice with rVSVΔG/SNVGPC was able to reduce viral RNA copy numbers in the blood and lungs of directly infected animals. More importantly, vaccination, either intramuscularly or orally, significantly reduced the number of transmission events in a SNV transmission model compared with control animals. This provides a proof-of-concept in which oral vaccination of deer mice results in protection against acquiring the virus following direct contact with infected deer mice. Further development of bait style vaccines for SNV or other rodent-borne viruses could provide an effective means of reducing disease burden.

Sin Nombre 病毒 (SNV) 是北美汉坦病毒心肺综合征 (HCPS) 的主要原因，这是一种致死率高的严重呼吸道疾病。SNV 由手足鲈, 或鹿小鼠, 人类感染是在小鼠排泄物或分泌物中吸入雾化病毒后发生的, 通常在家庭周围环境中。目前还没有 FDA 批准的针对 SNV 或任何其他汉坦病毒的疫苗或疗法，因此预防感染是减轻 HCPS 疾病负担的重要手段。预防 HCPS 病例的一种方法是通过诱饵疫苗接种防止病毒在啮齿动物宿主种群中传播。然而，与针对较大物种的疫苗不同，啮齿动物传播病毒的诱饵式疫苗尚未在该领域使用。在这里，我们利用表达 SNV 糖蛋白前体 (rVSVΔG/SNVGPC) 的重组水泡性口炎病毒来阻止 SNV 传播。用 rVSVΔG/SNVGPC 对鹿小鼠进行疫苗接种能够减少直接感染动物血液和肺中的病毒 RNA 拷贝数。更重要的是，与对照动物相比，肌肉内或口服疫苗接种显着减少了 SNV 传播模型中传播事件的数量。这提供了一个概念验证，其中鹿鼠的口服疫苗接种可防止在与受感染的鹿鼠直接接触后获得病毒。进一步开发针对 SNV 或其他啮齿动物传播病毒的诱饵式疫苗可以提供减少疾病负担的有效手段。这提供了一个概念验证，其中鹿鼠的口服疫苗接种可防止在与受感染的鹿鼠直接接触后获得病毒。进一步开发针对 SNV 或其他啮齿动物传播病毒的诱饵式疫苗可以提供减少疾病负担的有效手段。这提供了一个概念验证，其中鹿鼠的口服疫苗接种可防止在与受感染的鹿鼠直接接触后获得病毒。进一步开发针对 SNV 或其他啮齿动物传播病毒的诱饵式疫苗可以提供减少疾病负担的有效手段。