Frontiers in Cell and Developmental Biology ( IF 4.6 ) Pub Date : 2020-06-16 , DOI: 10.3389/fcell.2020.00582 Elena V Mitroshina 1 , Roman S Yarkov 1 , Tatiana A Mishchenko 1, 2 , Victoria G Krut' 1 , Maria S Gavrish 1 , Ekaterina A Epifanova 1 , Alexey A Babaev 1 , Maria V Vedunova 1
Alzheimer’s disease (AD) is a widespread chronic neurodegenerative pathology characterized by synaptic dysfunction, partial neuronal death, cognitive decline and memory impairments. The major hallmarks of AD are extracellular senile amyloid plaques formed by various types of amyloid proteins (Aβ) and the formation and accumulation of intracellular neurofibrillary tangles. However, there is a lack of relevant experimental models for studying changes in neural network activity, the features of intercellular signaling or the effects of drugs on the functional activity of nervous cells during AD development. In this work, we examined two experimental models of amyloidopathy using primary hippocampal cultures. The first model involves the embryonic brains of 5xFAD mice; the second uses chronic application of amyloid beta 1-42 (Aβ1-42). The model based on primary hippocampal cells obtained from 5xFAD mice demonstrated changes in spontaneous network calcium activity characterized by a decrease in the number of cells exhibiting Ca2+ activity, a decrease in the number of Ca2+ oscillations and an increase in the duration of Ca2+ events from day 21 of culture development
中文翻译:
脑源性神经营养因子(BDNF)可以在体外维持β-淀粉样变性模型中神经网络的功能完整性。
阿尔茨海默氏病(AD)是一种广泛的慢性神经退行性病变,其特征在于突触功能障碍,部分神经元死亡,认知能力下降和记忆障碍。AD的主要特征是由各种类型的淀粉样蛋白(Aβ)形成的细胞外老年淀粉样斑块以及细胞内神经原纤维缠结的形成和积累。然而,缺乏相关的实验模型来研究神经网络活动的变化,细胞间信号的特征或药物对AD发展过程中神经细胞功能活性的影响。在这项工作中,我们检查了使用原代海马培养物的两种淀粉样变性实验模型。第一个模型涉及5xFAD小鼠的胚胎脑。第二种是长期使用淀粉样蛋白1-1-4(Aβ1-42)。从培养开始的第21天起2+活性,Ca 2+振荡次数减少和Ca 2+事件持续时间增加