The aim of the present study was to investigate the effect of dietary supplementation of royal jelly (RJ) on liquid and frozen storage of rooster spermatozoa. Twenty‐five 30‐week‐old of Mazandaran native breeder roosters were randomly divided into five treatments (n = 5 roosters/group). Experimental treatments are designed to include a control group and various levels (0.0 (RJ0), 100 (RJ100), 200 (RJ200), 300 (RJ300) mg kg⁻¹ BW⁻¹) of royal jelly (RJ) that were fed to the roosters using force‐feed method. The percentage of forward progressive motility, abnormal spermatozoa, membrane integrity and viability of spermatozoa evaluated after 24 and 48 hr of cooling (at 4°C) and after the freeze–thawing process. Also, mitochondrial activity and DNA fragmentation in fresh (24 hr) and post‐thawed spermatozoa were assessed. The result of this study showed that the spermatozoa forward progressive motility, abnormality, membrane integrity, and viability were improved by the RJ100 group compared to the other groups after 24 and 48 hr storage period at 4°C. The percentage of membrane integrity and forward progressive motility after freeze–thawing in the RJ100 group was significantly higher than the other groups, and the percentage of abnormal spermatozoa was lower. A significant decrease in semen quality parameters was seen after 24 and 48 hr of refrigeration, but there was no observed change between 2 and 24 hr in the RJ100. The viability percentage of spermatozoa in both RJ100 and RJ200 groups was not different. Moreover, after freeze–thawing, DNA integrity and mitochondrial activity in the RJ100 group were significantly higher than the other groups. According to our results, feeding of RJ at 100 mg kg⁻¹ BW⁻¹ to the roosters was improved spermatozoa characteristics during liquid and cryopreservation conditions.

中文翻译：

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内服蜂王浆可改善公鸡精子的保存。

本研究的目的是调查蜂王浆 (RJ) 膳食补充剂对公鸡精子液体和冷冻储存的影响。25 只 30 周龄的马赞达兰本地种鸡被随机分为 5 个处理（n  = 5 只公鸡/组）。实验处理旨在包括一个对照组和各种水平（0.0 (RJ0)、100 (RJ100)、200 (RJ200)、300 (RJ300) mg kg ^-1  BW ^-1) 使用强制喂食方法喂给公鸡的蜂王浆 (RJ)。在冷却 24 和 48 小时（4°C）和冻融过程后评估的前向运动、异常精子、膜完整性和精子活力的百分比。此外，还评估了新鲜（24 小时）和解冻后精子中的线粒体活性和 DNA 片段化。这项研究的结果表明，在 4°C 下储存 24 和 48 小时后，与其他组相比，RJ100 组的精子向前进行性运动、异常、膜完整性和活力得到改善。RJ100组冻融后膜完整性和前向运动百分比显着高于其他组，异常精子百分比较低。在冷藏 24 和 48 小时后，精液质量参数显着下降，但 RJ100 在 2 和 24 小时之间没有观察到变化。RJ100 和 RJ200 组的精子存活率没有差异。此外，冻融后，RJ100组的DNA完整性和线粒体活性显着高于其他组。根据我们的结果，以 100 mg/kg 喂食 RJ^-1  BW ^-1在液体和冷冻保存条件下改善了公鸡的精子特性。