Benzenoids are compounds associated with floral and fruity flavours in flowers, fruits and leaves and present a role in hormonal signalling in plants. These molecules are produced by the phenyl ammonia lyase pathway. However, some yeasts can also synthesize them from aromatic amino acids using an alternative pathway that remains unknown. Hanseniaspora vineae can produce benzenoids at levels up to two orders of magnitude higher than Saccharomyces species, so it is a model microorganism for studying benzenoid biosynthesis pathways in yeast. According to their genomes, several enzymes have been proposed to be involved in a mandelate pathway similar to that described for some prokaryotic cells. Among them, the ARO10 gene product could present benzoylformate decarboxylase activity. This enzyme catalyses the decarboxylation of benzoylformate into benzaldehyde at the end of the mandelate pathway in benzyl alcohol formation. Two homologous genes of ARO10 were found in the two sequenced H. vineae strains. In this study, nine other H. vineae strains were analysed to detect the presence and per cent homology of ARO10 sequences by PCR using specific primers designed for this species. Also, the copy number of the genes was estimated by quantitative PCR. To verify the relation of ARO10 with the production of benzyl alcohol during fermentation, a deletion mutant in the ARO10 gene of Saccharomyces cerevisiae was used. The two HvARO10 paralogues were analysed and compared with other α‐ketoacid decarboxylases at the sequence and structural level.

中文翻译：

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遗传和转录组学证据表明 ARO10 基因参与酵母的苯类生物合成。

苯类化合物是与花、果实和叶子中的花香和果味有关的化合物，在植物的激素信号传导中发挥作用。这些分子由苯氨裂解酶途径产生。然而，一些酵母也可以使用仍然未知的替代途径从芳香族氨基酸合成它们。Hanseniaspora vineae可以产生比酵母菌高两个数量级的苯类，因此它是研究酵母苯类生物合成途径的模型微生物。根据他们的基因组，已经提出几种酶参与类似于某些原核细胞所描述的扁桃体途径。其中，ARO10基因产物可呈现苯甲酰甲酸脱羧酶活性。该酶在苯甲醇形成的扁桃酸途径末端催化苯甲酰甲酸酯脱羧为苯甲醛。两个同源基因ARO10两个测序发现H. vineae株。在这项研究中，等九级H. vineae进行分析的菌株，以检测存在和每百分之同源ARO10利用设计用于该物种特异性引物序列通过PCR。此外，通过定量 PCR 估计基因的拷贝数。要验证的关系ARO10具有生产苄醇的发酵过程中，在缺失突变体ARO10使用酿酒酵母的基因。分析了两个 Hv ARO10旁系同源物，并在序列和结构水平上与其他 α-酮酸脱羧酶进行了比较。