In this study, the effects of cold plasma and plasma‐activated medium (PAM) are investigated through different treatment times, different intervals between treatment and analysis, and synthesis of helium gas and helium + 0.5% oxygen. The viability of two cancer cell lines including Hela and MDA‐MB‐231, which are related to cervix and breast cancers, respectively, is investigated using the 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide assay. To study the effect of plasma on normal cells, the corresponding method is used for examination of the normal human fibroblasts. H₂O₂ showed a higher concentration after direct‐plasma treatment than PAM treatment. Caspase 3, caspase 8, and Bax proteins and the relative Bax/Bcl‐2 ratio are compared in both the direct‐plasma and PAM methods. The results show that the expression level of apoptotic proteins caspase 3 and caspase 8 in direct‐plasma treatment is higher than that in PAM treatment, and the level of relative Bcl‐2/Bax ratio is increased, demonstrating the induction of the apoptosis mechanism.

中文翻译：

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直接冷大气血浆和血浆激活的培养基对乳腺癌和子宫颈癌细胞的影响

在本研究中，通过不同的处理时间，不同的处理和分析间隔以及氦气和氦气+ 0.5％氧气的合成，研究了冷等离子体和等离子体激活介质（PAM）的影响。使用3- [4,5-二甲基噻唑-2-基] -2-，5-二苯基研究了两种分别与宫颈癌和乳腺癌相关的癌细胞系Hela和MDA-MB-231的生存力溴化四氮唑测定。为了研究血浆对正常细胞的作用，使用相应的方法检查正常人的成纤维细胞。H ₂ O ₂血浆直接血浆处理后的浓度高于PAM处理。在直接血浆和PAM方法中都比较了Caspase 3，Caspase 8和Bax蛋白以及相对Bax / Bcl-2比率。结果表明，直接血浆处理中凋亡蛋白caspase 3和caspase 8的表达水平高于PAM处理，并且相对Bcl-2 / Bax比的水平增加，证明了其凋亡机制的诱导。