Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance.,Macromolecular Rapid Communications

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Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance.
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2020-07-08 , DOI: 10.1002/marc.202000295
Shuang Wang ₁ , Mariah Sarwat ₁ , Peng Wang ₁ , Denver C Surrao ₂ , Damien G Harkin ₁ , James A St John ₂ , Eleonore C L Bolle ₁ , Aurelien Forget ₃ , Paul D Dalton ₄ , Tim R Dargaville ₁

Affiliation

A method is reported for making hollow channels within hydrogels decorated with cell‐adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide‐thiol chemistry, which are backfilled with a cysteine‐containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell‐guides, reducing the amount of required peptide 20‐fold when compared to bulk functionalization. The power of this approach is highlighted by successfully using these patterned hydrogels without active perfusion to guide fibroblasts and olfactory ensheathing cells—the latter having unique potential in neural repair therapies.

中文翻译：

具有细胞粘附肽修饰通道壁的水凝胶，可用于细胞引导。

据报道，有一种方法可以在水凝胶中仅在通道表面上用细胞粘附肽修饰的空心通道。使用不同直径的牺牲纤维在与马来酰亚胺-硫醇化学交联的聚乙二醇水凝胶中引入通道，并在通道中填充含半胱氨酸的肽溶液，该溶液以良好的空间效率与内腔结合。当用作细胞指导时，这仅允许在需要水凝胶的区域进行肽构图，与批量功能化相比，所需的肽量减少了20倍。成功地使用这些图案化的水凝胶而不进行主动灌注来引导成纤维细胞和嗅鞘细胞（后者在神经修复疗法中具有独特的潜力），就突出了这种方法的强大功能。

更新日期：2020-08-06

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